Opsonophagocytosis of Fluorescent Polystyrene Beads Coupled to Neisseria Meningitidis Serogroup A, C, Y, or W135 Polysaccharide Correlates with Serum Bactericidal Activity

Overview

Journal Clin Diagn Lab Immunol

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2002 Mar 5

PMID 11874898

Citations 8

Authors

Joseph Martinez

Tamara Pilishvili

Suzanne Barnard

Joseph Caba

Willie Spear

Sandra Romero-Steiner

George M Carlone

Affiliations

Soon will be listed here.

Abstract

We developed a polysaccharide-specific flow cytometric opsonophagocytic assay (OPA) for the simultaneous measurement of functional antibody to Neisseria meningitidis serogroups A, C, Y, and W135. OPA titers significantly correlated with serum bactericidal assay titers for all serogroups tested (mean r = 0.96; P < 0.001). OPA could be used in meningococcal vaccine evaluation.

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