Low Molecular Mass Dinitrosyl Nonheme-iron Complexes Up-regulate Noradrenaline Release in the Rat Tail Artery

Overview

Journal BMC Pharmacol

Publisher Biomed Central

Specialty Pharmacology

Date 2002 Mar 2

PMID 11872148

Citations 1

Authors

Andrei L Kleschyov

Gilles Hubert

Thomas Munzel

Jean-Claude Stoclet

Bernard Bucher

Affiliations

Soon will be listed here.

Abstract

Background: Dinitrosyl nonheme-iron complexes can appear in cells and tissues overproducing nitric oxide. It is believed that due to their chemical nature these species may be implicated in certain pathophysiological events. We studied the possible role of low molecular mass dinitrosyl iron complexes in the control of noradrenaline release in electrically stimulated rat tail artery.

Results: A model complex, dinitrosyl-iron-thiosulfate (at 1-10 microM) produced a concentration-dependent enhancement of electrical field stimulated [3H]noradrenaline release (up to 2 fold). At the same time, dinitrosyl-iron-thiosulfate inhibited neurogenic vasoconstriction, consistent with its nitric oxide donor properties. A specific inhibitor of cyclic GMP dependent protein kinase, Rp-8pCPT-cGMPS, partially inhibited the effect of dinitrosyl-iron-thiosulfate on neurogenic vasoconstriction, but not on [3H]noradrenaline release. Another model complex, dinitrosyl-iron-cysteine (at 3 microM) elicited similar responses as dinitrosyl-iron-thiosulfate. Conventional NO and NO+ donors such as sodium nitroprusside, S-nitroso-L-cysteine or S-nitroso-glutathione (at 10 microM) had no effect on [3H]noradrenaline release, though they potently decreased electrically-induced vasoconstriction. The "false complex", iron(II)-thiosulfate showed no activity.

Conclusions: Low molecular mass iron dinitrosyl complexes can up-regulate the stimulation-evoked release of vascular [3H]noradrenaline, apparently independently of their NO donor properties. This finding may have important implications in inflammatory tissues.

Citing Articles

Iron nitrosyl complexes as models for biological nitric oxide transfer reagents.

Chiang C, Darensbourg M J Biol Inorg Chem. 2006; 11(3):359-70.

PMID: 16520978 DOI: 10.1007/s00775-006-0084-y.

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