Recombinase-directed Plant Transformation for the Post-genomic Era

Overview

Journal Plant Mol Biol

Date 2002 Feb 28

PMID 11860209

Citations 44

Authors

David W Ow

Affiliations

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Abstract

Plant genomics promises to accelerate genetic discoveries for plant improvements. Machine-driven technologies are ushering in gene structural and expressional data at an unprecedented rate. Potential bottlenecks in this crop improvement process are steps involving plant transformation. With few exceptions, genetic transformation is an obligatory final step by which useful traits are engineered into plants. In addition, transgenesis is most often needed to confirm gene function, after deductions made through comparative genomics, expression profiles, and mutation analysis. This article reviews the use of recombinase systems to deliver DNA more efficiently into the plant genome.

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References

Lyznik L, Rao K, Hodges T . FLP-mediated recombination of FRT sites in the maize genome. Nucleic Acids Res. 1996; 24(19):3784-9. PMC: 146161. DOI: 10.1093/nar/24.19.3784. View

Choi S, Begum D, Koshinsky H, Ow D, Wing R . A new approach for the identification and cloning of genes: the pBACwich system using Cre/lox site-specific recombination. Nucleic Acids Res. 2000; 28(7):E19. PMC: 102802. DOI: 10.1093/nar/28.7.e19. View

Iyer L, Kumpatla S, Chandrasekharan M, Hall T . Transgene silencing in monocots. Plant Mol Biol. 2000; 43(2-3):323-46. DOI: 10.1023/a:1006412318311. View

Offringa R, Franke-van Dijk M, de Groot M, van den Elzen P, Hooykaas P . Nonreciprocal homologous recombination between Agrobacterium transferred DNA and a plant chromosomal locus. Proc Natl Acad Sci U S A. 1993; 90(15):7346-50. PMC: 47134. DOI: 10.1073/pnas.90.15.7346. View

Lyznik L, Hirayama L, Rao K, Abad A, Hodges T . Heat-inducible expression of FLP gene in maize cells. Plant J. 1995; 8(2):177-86. DOI: 10.1046/j.1365-313x.1995.08020177.x. View

Thomason L, Calendar R, Ow D . Gene insertion and replacement in Schizosaccharomyces pombe mediated by the Streptomyces bacteriophage phiC31 site-specific recombination system. Mol Genet Genomics. 2001; 265(6):1031-8. DOI: 10.1007/s004380100498. View

Qin M, Bayley C, Stockton T, Ow D . Cre recombinase-mediated site-specific recombination between plant chromosomes. Proc Natl Acad Sci U S A. 1994; 91(5):1706-10. PMC: 43232. DOI: 10.1073/pnas.91.5.1706. View

Lloyd A, Davis R . Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum. Mol Gen Genet. 1994; 242(6):653-7. DOI: 10.1007/BF00283419. View

Bethke B, Sauer B . Segmental genomic replacement by Cre-mediated recombination: genotoxic stress activation of the p53 promoter in single-copy transformants. Nucleic Acids Res. 1997; 25(14):2828-34. PMC: 146822. DOI: 10.1093/nar/25.14.2828. View

10.

Araki K, Araki M, Yamamura K . Targeted integration of DNA using mutant lox sites in embryonic stem cells. Nucleic Acids Res. 1997; 25(4):868-72. PMC: 146486. DOI: 10.1093/nar/25.4.868. View

11.

Sonti R, Tissier A, Wong D, Viret J, Signer E . Activity of the yeast FLP recombinase in Arabidopsis. Plant Mol Biol. 1995; 28(6):1127-32. DOI: 10.1007/BF00032673. View

12.

Lorbach E, Christ N, Schwikardi M, Droge P . Site-specific recombination in human cells catalyzed by phage lambda integrase mutants. J Mol Biol. 2000; 296(5):1175-81. DOI: 10.1006/jmbi.2000.3532. View

13.

Russell S, Hoopes J, Odell J . Directed excision of a transgene from the plant genome. Mol Gen Genet. 1992; 234(1):49-59. DOI: 10.1007/BF00272344. View

14.

Onouchi H, Yokoi K, Machida C, Matsuzaki H, Oshima Y, Matsuoka K . Operation of an efficient site-specific recombination system of Zygosaccharomyces rouxii in tobacco cells. Nucleic Acids Res. 1991; 19(23):6373-8. PMC: 329180. DOI: 10.1093/nar/19.23.6373. View

15.

Corneille S, Lutz K, Svab Z, Maliga P . Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system. Plant J. 2001; 27(2):171-8. DOI: 10.1046/j.1365-313x.2001.01068.x. View

16.

Alonso J, Weise F, Rojo F . The Bacillus subtilis histone-like protein Hbsu is required for DNA resolution and DNA inversion mediated by the beta recombinase of plasmid pSM19035. J Biol Chem. 1995; 270(7):2938-45. DOI: 10.1074/jbc.270.7.2938. View

17.

Onouchi H, Nishihama R, Kudo M, Machida Y, Machida C . Visualization of site-specific recombination catalyzed by a recombinase from Zygosaccharomyces rouxii in Arabidopsis thaliana. Mol Gen Genet. 1995; 247(6):653-60. DOI: 10.1007/BF00290396. View

18.

Sugita K, Kasahara T, Matsunaga E, Ebinuma H . A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J. 2000; 22(5):461-9. DOI: 10.1046/j.1365-313x.2000.00745.x. View

19.

Sieburth L, Drews G, Meyerowitz E . Non-autonomy of AGAMOUS function in flower development: use of a Cre/loxP method for mosaic analysis in Arabidopsis. Development. 1998; 125(21):4303-12. DOI: 10.1242/dev.125.21.4303. View

20.

Srivastava V, Ow D . Single-copy primary transformants of maize obtained through the co-introduction of a recombinase-expressing construct. Plant Mol Biol. 2001; 46(5):561-6. DOI: 10.1023/a:1010646100261. View