The Use of Calcium Blockers to Study Biochemical Behaviour of Saccharomyces Cerevisiae Cells

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2002 Feb 22

PMID 11855734

Citations 2

Authors

R de Souza Pereira

Affiliations

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Abstract

Calcium channel blockers, currently used in clinical medicine, from the dihydropyridine class (nifedipine and nimodipine) were used to study biochemical behaviour of Saccharomyces cerevisiae cells. At a concentration of 200 microM, both drugs partially blocked calcium uptake from an extracellular medium. On the other hand, nifedipine (300 microM) induced a collapse of the membrane potential (delta psi) of these cells, while nimodipine, at the same concentration, produced a similar but less intense effect. Probably, the small difference in the chemical structure between these drugs may explain the same effect with different intensity. With these experiments was possible to observe that these microorganisms have type 'L' calcium channels.

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References

Castilho R, Pereira R, Vercesi A . Protective effect of safranine on the mitochondrial damage induced by Fe(II)citrate: comparative study with trifluoperazine. Eur J Drug Metab Pharmacokinet. 1996; 21(1):17-21. DOI: 10.1007/BF03190273. View

Pereira R, Hermes-Lima M . Can trifluoperazine protect mitochondria against reactive oxygen species-induced damage?. Eur J Drug Metab Pharmacokinet. 1996; 21(4):281-4. DOI: 10.1007/BF03189728. View

Pereira R . The use of baker's yeast in the generation of asymmetric centers to produce chiral drugs and others compounds. Crit Rev Biotechnol. 1998; 18(1):25-83. DOI: 10.1080/0738-859891224211. View

Pereira R, Bertocchi A, Vercesi A . Protective effect of trifluoperazine on the mitochondrial damage induced by Ca2+ plus prooxidants. Biochem Pharmacol. 1992; 44(9):1795-801. DOI: 10.1016/0006-2952(92)90074-s. View

Pereira R . Baker's yeast. Some biochemical aspects and their influence in biotransformations. Appl Biochem Biotechnol. 1995; 55(2):123-32. DOI: 10.1007/BF02783553. View

Wolkowicz P . Dissociation between mitochondria calcium ion release and pyridine nucleotide oxidation. J Biol Chem. 1980; 255(21):10348-53. View

Schwartz A, McKenna E, Vaghy P . Receptors for calcium antagonists. Am J Cardiol. 1988; 62(11):3G-6G. DOI: 10.1016/0002-9149(88)90024-0. View

Tsien R, Nilius B . Cardiac calcium currents at the level of single channels. Experientia. 1987; 43(11-12):1169-72. DOI: 10.1007/BF01945517. View

Catterall W . Structure and function of voltage-sensitive ion channels. Science. 1988; 242(4875):50-61. DOI: 10.1126/science.2459775. View

10.

Catterall W, Seagar M, Takahashi M . Molecular properties of dihydropyridine-sensitive calcium channels in skeletal muscle. J Biol Chem. 1988; 263(8):3535-8. View

11.

Eilam Y . Studies on calcium efflux in the yeast Saccharomyces cerevisiae. Microbios. 1982; 35(140):99-110. View

12.

de Souza Pereira R . Comparison of biochemical effects produced by calcium ions and by monomers of polyacrylamide (acrylamide and bisacrylamide) on strains of Saccharomyces cerevisiae used for production of chiral synthons. Mol Cell Biochem. 1998; 178(1-2):33-40. DOI: 10.1023/a:1006834404049. View

13.

Tsien R, Lipscombe D, Madison D, Bley K, Fox A . Multiple types of neuronal calcium channels and their selective modulation. Trends Neurosci. 1988; 11(10):431-8. DOI: 10.1016/0166-2236(88)90194-4. View

14.

YOUATT J . Calcium and microorganisms. Crit Rev Microbiol. 1993; 19(2):83-97. DOI: 10.3109/10408419309113524. View