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Babesia Bovis Merozoite Surface Antigen 1 and Rhoptry-associated Protein 1 Are Expressed in Sporozoites, and Specific Antibodies Inhibit Sporozoite Attachment to Erythrocytes

Overview
Journal Infect Immun
Date 2002 Feb 21
PMID 11854249
Citations 25
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Abstract

We examined Babesia bovis sporozoites for the expression of two molecules, merozoite surface antigen 1 (MSA-1) and rhoptry-associated protein 1 (RAP-1), that are postulated to be involved in the invasion of host erythrocytes. Both MSA-1 and RAP-1 were transcribed and expressed in infectious sporozoites. Importantly, monospecific MSA-1 and RAP-1 antisera each inhibited sporozoite invasion of erythrocytes in vitro. This is the first identification of antigens expressed in Babesia sp. sporozoites and establishes that, at least in part, sporozoites and merozoites share common targets of antibody mediated inhibition of erythrocyte invasion.

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References
1.
Jasmer D, Reduker D, Hines S, Perryman L, McGuire T . Surface epitope localization and gene structure of a Babesia bovis 44-kilodalton variable merozoite surface antigen. Mol Biochem Parasitol. 1992; 55(1-2):75-83. DOI: 10.1016/0166-6851(92)90128-7. View

2.
Sam-Yellowe T . Rhoptry organelles of the apicomplexa: Their role in host cell invasion and intracellular survival. Parasitol Today. 1996; 12(8):308-16. DOI: 10.1016/0169-4758(96)10030-2. View

3.
Suarez C, Palmer G, Jasmer D, Hines S, Perryman L, Mcelwain T . Characterization of the gene encoding a 60-kilodalton Babesia bovis merozoite protein with conserved and surface exposed epitopes. Mol Biochem Parasitol. 1991; 46(1):45-52. DOI: 10.1016/0166-6851(91)90197-e. View

4.
Hines S, Palmer G, Jasmer D, Goff W, Mcelwain T . Immunization of cattle with recombinant Babesia bovis merozoite surface antigen-1. Infect Immun. 1995; 63(1):349-52. PMC: 172999. DOI: 10.1128/iai.63.1.349-352.1995. View

5.
Mahoney D, Wright I, Ketterer P . Babesia argentina: the infectivity and immunogenicity of irradiated blood parasites for splenectomized calves. Int J Parasitol. 1973; 3(2):209-17. DOI: 10.1016/0020-7519(73)90026-x. View