Complete Inhibition of Tobamovirus Multiplication by Simultaneous Mutations in Two Homologous Host Genes

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2002 Feb 12

PMID 11836427

Citations 33

Authors

Takuya Yamanaka

Takahiro Imai

Rena Satoh

Arata Kawashima

Miki Takahashi

Kayo Tomita

Kenji Kubota

Tetsuo Meshi

Satoshi Naito

Masayuki Ishikawa

Affiliations

Soon will be listed here.

Abstract

The TOM1 gene of Arabidopsis thaliana encodes a putative multipass transmembrane protein which is necessary for the efficient multiplication of tobamoviruses. We have previously shown that mutations severely destructive to the TOM1 gene reduce tobamovirus multiplication to low levels but do not impair it completely. In this report, we subjected one of the tom1 mutants (tom1-1) to another round of mutagenesis and isolated a new mutant which did not permit a detectable level of tobamovirus multiplication. In addition to tom1-1, this mutant carried a mutation referred to as tom3-1. Positional cloning showed that TOM3 was one of two TOM1-like genes in Arabidopsis. Based on the similarity between the amino acid sequences of TOM1 and TOM3, together with the results of a Sos recruitment assay suggesting that both TOM1 and TOM3 bind tobamovirus-encoded replication proteins, we propose that TOM1 and TOM3 play parallel and essential roles in the replication of tobamoviruses.

Citing Articles

Genome-wide analysis of tobacco NtTOM1/TOM3 gene family and identification of NtTOM1a/ NtTOM3a response to tobacco mosaic virus.

Wang X, Bai Y, Shen Z, Zhang X, Cai C, Qiao C BMC Plant Biol. 2024; 24(1):942.

PMID: 39385089 PMC: 11465672. DOI: 10.1186/s12870-024-05632-1.

Engineered Resistance to Tobamoviruses.

Carr J Viruses. 2024; 16(7).

PMID: 39066170 PMC: 11281658. DOI: 10.3390/v16071007.

Plant Immunity against Tobamoviruses.

Zheng X, Li Y, Liu Y Viruses. 2024; 16(4).

PMID: 38675873 PMC: 11054417. DOI: 10.3390/v16040530.

Fine mapping and identification of two NtTOM2A homeologs responsible for tobacco mosaic virus replication in tobacco (Nicotiana tabacum L.).

Wang X, Shen Z, Li C, Bai Y, Li Y, Zhang W BMC Plant Biol. 2024; 24(1):67.

PMID: 38262958 PMC: 10807211. DOI: 10.1186/s12870-024-04744-y.

TOM1 family conservation within the plant kingdom for tobacco mosaic virus accumulation.

Zhang H, Hu Q Mol Plant Pathol. 2023; 24(11):1385-1399.

PMID: 37443447 PMC: 10576174. DOI: 10.1111/mpp.13375.

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