The Capsular Polysaccharide of Enterococcus Faecalis and Its Relationship to Other Polysaccharides in the Cell Wall

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 Feb 7

PMID 11830672

Citations 82

Authors

Lynn E Hancock

Michael S Gilmore

Affiliations

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Abstract

With the goal of identifying and characterizing traits of Enterococcus faecalis that play key roles in human disease, we identified an operon specifying synthesis of a capsular carbohydrate of the type most commonly expressed by clinical isolates. This surface-exposed carbohydrate consists of glycerol phosphate, glucose, and galactose residues, and its biosynthesis is encoded by a determinant that includes 11 ORFs. Insertional inactivation of genes in this pathway yielded mutants with enhanced susceptibility to phagocytic killing in vitro and compromised in the ability to persist in regional lymph nodes in vivo.

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