Chemotaxis of Ralstonia Eutropha JMP134(pJP4) to the Herbicide 2,4-dichlorophenoxyacetate

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2002 Feb 2

PMID 11823246

Citations 19

Authors

Andrew C Hawkins

Caroline S Harwood

Affiliations

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Abstract

Ralstonia eutropha JMP134(pJP4) and several other species of motile bacteria can degrade the herbicide 2,4-dichlorophenoxyacetate (2,4-D), but it was not known if bacteria could sense and swim towards 2,4-D by the process of chemotaxis. Wild-type R. eutropha cells were chemotactically attracted to 2,4-D in swarm plate assays and qualitative capillary assays. The chemotactic response was induced by growth with 2,4-D and depended on the presence of the catabolic plasmid pJP4, which harbors the tfd genes for 2,4-D degradation. The tfd cluster also encodes a permease for 2,4-D named TfdK. A tfdK mutant was not chemotactic to 2,4-D, even though it grew at wild-type rates on 2,4-D.

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