Maintenance and Characterization of an Epstein Barr Virus-infected CD56-negative T Cell Lymphoma

Overview

Journal Jpn J Cancer Res

Specialty Oncology

Date 2002 Jan 23

PMID 11802809

Authors

Izuru Maekawa

Hitoshi Satoh

Naoto Aoki

Yasuyuki Morishita

Norifumi Tsukamoto

Masamitsu Karasawa

Yasunobu Nonaka

Mami Shiota

Yoshihisa Nojima

Shigeo Mori

Affiliations

Soon will be listed here.

Abstract

T cell lymphoma carrying Epstein Barr virus (EBV(+) TL) is very rare among Western countries while it is much more common among Japanese. Here we report an EBV(+) TL which has been maintained for years by the use of mice with severe combined immune deficiency (SCID) mice. Lymphoma was obtained from a 55-year-old male suffering from oculomotor nerve palsy and lymphadenopathy. A small piece of biopsied tumor was transplanted into SCID mice and the lymphoma has been maintained for over 3 years with passages every 2 - 3 weeks. The maintained lymphoma, termed as TMS24, and the original lymphoma cells showed identical phenotype and genotype, including diffuse medium-sized cell morphology lacking granules, suppressor / cytotoxic immunophenotype and identical T cell receptor beta-chain gene rearrangement mode. Further, both were shown to carry an identical EBV clone in terms of the number of terminal repeats and the latency II-type restricted gene expression profile. Cytogenetically, TMS24 retained two characteristic chromosomal translocations of t(1;18)(q32;q21) and t(6;12)(p21;q24). Since only one cell line with such characters has been reported previously, TMS24 should be useful for detailed analysis of EBV(+) TL.

References

Shikano T, Arioka H, Kobayashi R, Naito H, Ishikawa Y . Jumping translocations of 1q in Burkitt lymphoma and acute nonlymphocytic leukemia. Cancer Genet Cytogenet. 1993; 71(1):22-6. DOI: 10.1016/0165-4608(93)90198-u. View

DAmore F, Johansen P, Houmand A, Weisenburger D, Mortensen L . Epstein-Barr virus genome in non-Hodgkin's lymphomas occurring in immunocompetent patients: highest prevalence in nonlymphoblastic T-cell lymphoma and correlation with a poor prognosis. Danish Lymphoma Study Group, LYFO. Blood. 1996; 87(3):1045-55. View

Cheng A, Su I, Chen Y, Uen W, Wang C . Characteristic clinicopathologic features of Epstein-Barr virus-associated peripheral T-cell lymphoma. Cancer. 1993; 72(3):909-16. DOI: 10.1002/1097-0142(19930801)72:3<909::aid-cncr2820720341>3.0.co;2-o. View

Jones J, Shurin S, Abramowsky C, Tubbs R, Sciotto C, Wahl R . T-cell lymphomas containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infections. N Engl J Med. 1988; 318(12):733-41. DOI: 10.1056/NEJM198803243181203. View

Macon W, Williams M, Greer J, Hammer R, Glick A, Collins R . Natural killer-like T-cell lymphomas: aggressive lymphomas of T-large granular lymphocytes. Blood. 1996; 87(4):1474-83. View

Chen H, Lee J, Wang Y, Huang D, Ambinder R, Hayward S . The Epstein-Barr virus latency BamHI-Q promoter is positively regulated by STATs and Zta interference with JAK/STAT activation leads to loss of BamHI-Q promoter activity. Proc Natl Acad Sci U S A. 1999; 96(16):9339-44. PMC: 17784. DOI: 10.1073/pnas.96.16.9339. View

Randerson J, Cawkwell L, Jack A, Lewis F, Johnson P, Evans P . Fluorescent polymerase chain reaction of a panel of CA repeats on chromosome 6 in the indolent phase of follicular centre cell lymphoma. Br J Cancer. 1996; 74(6):942-6. PMC: 2074751. DOI: 10.1038/bjc.1996.461. View

Fukayama M, Ibuka T, Hayashi Y, Ooba T, Koike M, Mizutani S . Epstein-Barr virus in pyothorax-associated pleural lymphoma. Am J Pathol. 1993; 143(4):1044-9. PMC: 1887050. View

Fan Y, Rizkalla K, William B, Engel C . Jumping translocations of 11q in acute myeloid leukemia and 1q in follicular lymphoma. Cancer Genet Cytogenet. 2000; 118(1):35-41. DOI: 10.1016/s0165-4608(99)00149-1. View

10.

Sasajima Y, Yamabe H, Kobashi Y, Hirai K, Mori S . High expression of the Epstein-Barr virus latent protein EB nuclear antigen-2 on pyothorax-associated lymphomas. Am J Pathol. 1993; 143(5):1280-5. PMC: 1887189. View

11.

Gray B, Bent-Williams A, Wadsworth J, Maiese R, Bhatia A, Zori R . Fluorescence in situ hybridization assessment of the telomeric regions of jumping translocations in a case of aggressive B-cell non-Hodgkin lymphoma. Cancer Genet Cytogenet. 1997; 98(1):20-7. DOI: 10.1016/s0165-4608(96)00409-8. View

12.

Tierney R, Steven N, Young L, Rickinson A . Epstein-Barr virus latency in blood mononuclear cells: analysis of viral gene transcription during primary infection and in the carrier state. J Virol. 1994; 68(11):7374-85. PMC: 237180. DOI: 10.1128/JVI.68.11.7374-7385.1994. View

13.

. The world health organization classification of malignant lymphomas in japan: incidence of recently recognized entities. Lymphoma Study Group of Japanese Pathologists. Pathol Int. 2000; 50(9):696-702. DOI: 10.1046/j.1440-1827.2000.01108.x. View

14.

Akasaka T, Miura I, Takahashi N, Akasaka H, Yonetani N, Ohno H . A recurring translocation, t(3;6)(q27;p21), in non-Hodgkin's lymphoma results in replacement of the 5' regulatory region of BCL6 with a novel H4 histone gene. Cancer Res. 1997; 57(1):7-12. View

15.

Suzuki R, Nakamura S . Malignancies of natural killer (NK) cell precursor: myeloid/NK cell precursor acute leukemia and blastic NK cell lymphoma/leukemia. Leuk Res. 1999; 23(7):615-24. DOI: 10.1016/s0145-2126(98)00194-5. View

16.

Harris N, Jaffe E, STEIN H, Banks P, Chan J, Cleary M . A revised European-American classification of lymphoid neoplasms: a proposal from the International Lymphoma Study Group. Blood. 1994; 84(5):1361-92. View

17.

Stoffel A, Rao P, Louie D, Krauter K, Liebowitz D, Koeppen H . Chromosome 18 breakpoint in t(11;18)(q21;q21) translocation associated with MALT lymphoma is proximal to BCL2 and distal to DCC. Genes Chromosomes Cancer. 1999; 24(2):156-9. View

18.

Kelleher C, Dreyfus D, Jones J, Gelfand E . EBV infection of T cells: potential role in malignant transformation. Semin Cancer Biol. 1996; 7(4):197-207. DOI: 10.1006/scbi.1996.0027. View

19.

Hamilton-Dutoit S, Pallesen G, Karkov J, Skinhoj P, Franzmann M, Pedersen C . Identification of EBV-DNA in tumour cells of AIDS-related lymphomas by in-situ hybridisation. Lancet. 1989; 1(8637):554-2. DOI: 10.1016/s0140-6736(89)90093-7. View

20.

Shiota M, Nakamura S, Ichinohasama R, Abe M, Akagi T, Takeshita M . Anaplastic large cell lymphomas expressing the novel chimeric protein p80NPM/ALK: a distinct clinicopathologic entity. Blood. 1995; 86(5):1954-60. View