Characterisation of Soy Isoflavones and Screening for Novel Malonyl Glycosides Using High-performance Liquid Chromatography-electrospray Ionisation-mass Spectrometry

Overview

Journal Phytochem Anal

Publisher Wiley

Specialties Biology
Chemistry

Date 2002 Jan 17

PMID 11793816

Citations 4

Authors

L Gu

W Gu

Affiliations

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Abstract

HPLC combined with electrospray ionisation (ESI)-MS and photodiode array detection has been employed to study the isoflavone components of soy. All of the known soy isoflavones separated by HPLC were identified and characterised, and three novel isoflavones were detected and screened out. These minor isoflavones were deduced to be isomers of 6"-O-malonyl isoflavone glycosides, based on the ESI-MS and UV data, in which the malonyl group is attached at a position other than the 6" position of the glycosyl moiety of the molecule. These novel malonyl glycosides are as thermally labile as the 6"-O-malonyl glycosides, being converted into known isoflavone glycosides after heating in aqueous ethanol. The advantages of HPLC-ESI-MS in detection of novel isoflavones from plant extracts are reviewed.

Citing Articles

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Profiling of flavonoid conjugates in Lupinus albus and Lupinus angustifolius responding to biotic and abiotic stimuli.

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