» Articles » PMID: 11792582

Nanoindentation Discriminates the Elastic Properties of Individual Human Bone Lamellae Under Dry and Physiological Conditions

Overview
Journal Bone
Date 2002 Jan 17
PMID 11792582
Citations 66
Authors
Affiliations
Soon will be listed here.
Abstract

Mechanical properties of single lamellae of human compact and trabecular bone tissue were measured with a combined atomic force microscopy (AFM) and nanoindentation technique. This combination allows for both characterization of bone surface topography and indentation of the bone extracellular matrix (ECM) with depths of between 100 and 600 nm. Four bone structural units (BSUs) were tested with 400 indents under dry conditions, and four BSUs with 160 indents were tested in a liquid cell under physiological conditions. A correspondence was established between the optical appearance of bone lamellae and the topography of the polished bone surface. The indentation modulus and hardness of bone ECM were investigated as a function of lamella type and indentation depth under wet and dry conditions. For low depth indents, thick lamellae showed a higher indentation modulus than thin lamellae. With increasing indentation depth, thick lamellae exhibited a significant decrease in indentation modulus and hardness, whereas, for thin lamellae, the effect of indentation depth was much less significant. These trends were similar for dry and physiological conditions and support compositional and/or ultrastructural differences between thick and thin lamellae.

Citing Articles

On the influence of structural and chemical properties on the elastic modulus of woven bone under healing.

Blazquez-Carmona P, Mora-Macias J, Pajares A, Marmol A, Reina-Romo E Front Bioeng Biotechnol. 2024; 12:1476473.

PMID: 39411059 PMC: 11473380. DOI: 10.3389/fbioe.2024.1476473.


Elasticity and material anisotropy of lamellar cortical bone in adult bovine tibia characterized via AFM nanoindentation.

Cisneros T, Sevostianov I, Drach B J Mech Behav Biomed Mater. 2023; 144:105992.

PMID: 37393887 PMC: 10467531. DOI: 10.1016/j.jmbbm.2023.105992.


Biomechanical Characteristics and Analysis Approaches of Bone and Bone Substitute Materials.

Niu Y, Du T, Liu Y J Funct Biomater. 2023; 14(4).

PMID: 37103302 PMC: 10146666. DOI: 10.3390/jfb14040212.


Smart Biomechanical Adaptation Revealed by the Structure of Ostrich Limb Bones.

Conti S, Sala G, Mateus O Biomimetics (Basel). 2023; 8(1).

PMID: 36975328 PMC: 10046004. DOI: 10.3390/biomimetics8010098.


A Near-Infrared Mechanically Switchable Elastomeric Film as a Dynamic Cell Culture Substrate.

Spiaggia G, Taladriz-Blanco P, Hengsberger S, Septiadi D, Geers C, Lee A Biomedicines. 2023; 11(1).

PMID: 36672538 PMC: 9855853. DOI: 10.3390/biomedicines11010030.