Characterization of a Human Cytomegalovirus with Phosphorylation Site Mutations in the Immediate-early 2 Protein

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2001 Dec 26

PMID 11752183

Citations 13

Authors

Julie A Heider

Yongjun Yu

Thomas Shenk

James C Alwine

Affiliations

Soon will be listed here.

Abstract

A human cytomegalovirus mutant (TNsubIE2P) was constructed with alanine substitutions of four residues (T27, S144, T233, and S234) previously shown to be phosphorylated in the immediate-early 2 (IE2) protein. This mutant grew as well as the wild type at both low and high multiplicities of infection. The mutant activated the major immediate-early, UL4, and UL44 promoters to similar levels, and with similar kinetics, as wild-type virus. However, the TNsubIE2P mutant virus transactivated an endogenous simian virus 40 early promoter 4 h earlier and to higher levels than the wild-type virus in infected human fibroblasts. The modification of the IE2 protein by SUMO-1 (i.e., its sumoylated state) was also examined.

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