Basic Polypeptides As Histone Models: Circular Dichroism of Complexes of Model Polypeptides with DNA

Overview

Journal Biophys Chem

Specialty Biochemistry

Date 1975 Jul 1

PMID 1174648

Citations 3

Authors

J SPONAR

I FRIC

K BLAHA

Affiliations

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Abstract

Circular dichroism (CD) was used to study the complexes of DNA (in 0.15M NaCl) with two polypeptides considered as models of the histone molecules. CD spectra in the region of DNA absorption were studied with respect to the concentration used for annealing and to the molecular weight and composition of the DNA used. The properties of supernatants after centrifugation of aggregated complexes were examined. The effect of selectively bound antibiotics (actinomycin D and netropsin) on CD sprectra of complexes was investigated. The induced CD of proflavine molecules bound to DNA in the various complexes was also studied. It was concluded that changes in the CD spectra of DNA in complexes with the polypeptides are due to the formation of chiral superstructures, even if some conformational changes of DNA molecules themselves may also be decisive in some cases. The superstructure is affected by the composition of DNA, the role of (G + C) rich segments being particularly important.

Citing Articles

Basic polypeptides as histone models. Effect of conformation, base composition and methylation of nucleic acids on the interaction with H1 and histone models and on the circular dichroism of complexes.

SPONAR J, DOSKOCIL J, BLAHA K, Zimmer C Nucleic Acids Res. 1976; 3(2):507-21.

PMID: 1257058 PMC: 342919. DOI: 10.1093/nar/3.2.507.

Conformation and reactivity of DNA in the complex with protein. IV. Circular dichroism of poly-L-histidine model complexes with DNA polymers and specificity of the interaction.

Burchkardt G, Zimmer G, Luck G Nucleic Acids Res. 1976; 3(3):561-80.

PMID: 5706 PMC: 342925. DOI: 10.1093/nar/3.3.561.

Conformation and reactivity of DNA in the complex with proteins. III. Helix-coil transition and conformational studies of model complexes of DNA's with poly-L-histidine.

Burckhardt G, Zimmer C, Luck G Nucleic Acids Res. 1976; 3(3):537-59.

PMID: 5705 PMC: 342924. DOI: 10.1093/nar/3.3.537.