Domain Interactions on the Ntr Signal Transduction Pathway: Two-hybrid Analysis of Mutant and Truncated Derivatives of Histidine Kinase NtrB

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2001 Dec 14

PMID 11741861

Citations 15

Authors

Isabel Martinez-Argudo

Paloma Salinas

Rafael Maldonado

Asuncion Contreras

Affiliations

Soon will be listed here.

Abstract

We have used the yeast two-hybrid system to analyze protein-protein interactions mediated by domains of regulatory proteins of the ntr signal transduction system, including interactions among NtrB derivatives and their interactions with NtrC and PII from Klebsiella pneumoniae. Interactions took place only between proteins or protein domains belonging to the ntr signal transduction system and not between proteins or domains from noncognate regulators. NtrB and its transmitter domain, but not NtrC, CheA, or the cytoplasmic C terminus of EnvZ, interacted with PII. In addition, interaction of NtrB with NtrC, but not with PII, depended on the histidine phosphotransfer domain. Point mutation A129T, diminishing the NtrC phosphatase activity of NtrB, affected the strength of the signals between NtrC and the transmitter module of NtrB but had no impact on PII signals, suggesting that A129T prevents the conformational change needed by NtrB to function as a phosphatase for NtrC, rather than disturbing binding to PII.

Citing Articles

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