Spontaneous Tension Oscillations in Guinea-pig Atrial Trabeculae

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1975 Jul 9

PMID 1172235

Citations 14

Authors

H G GLITSCH

L Pott

Affiliations

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Abstract

Spontaneous tension oscillations have been recorded from intact guinea-pig auricular trabeculae bathed in Na-poor and/or Ca-rich solutions. The frequency of these oscillations and that of after-contractions (oscillations following an electrically induced contraction) evoked under identical experimental conditions was the same (33 degrees C). The amplitude of the oscillations rose when the [Ca2+]0/[Na+]0(2)-ratio or the intracellular Na-concentration was increased. When the increase of the [Ca2+]0/[Na+]0(2)-ratio was relatively small, tension oscillations only occured after a period of electrical stimulation. The oscillation-frequency increased slightly in media containing 70 instead of 5.4 mM KCl. MnCl2 (3 mM) did not affect either the amplitude or the frequency of the oscillations. Caffeine (0.5-2.5 mM) decreased the amplitude and enhanced the frequency of the oscillations. After-contractions were diminished and, at higher concentrations, abolished. It is demonstrated that the membrane potential does not participate in the process causing the tension oscillations. An increased [Ca2+]i is a prerequisite for the occurrence of these oscillations. Characteristics of intracellular Ca-movement probably determine the amplitude and frequency of the spontaneous oscillations of tension.

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References

Ford L, PODOLSKY R . Intracellular calcium movements in skinned muscle fibres. J Physiol. 1972; 223(1):21-33. PMC: 1331430. DOI: 10.1113/jphysiol.1972.sp009831. View

Verdonck F, Busselen P, Carmeliet E . Ca-action potentials and contractions of heart muscle in Na-free solutions. Influence of caffeine. Arch Int Physiol Biochim. 1972; 80(1):167-9. View

Kaufmann R, Homburger H, Tritthart H . [Specific inhibition of relaxation in mammalian myocardium at low temperatures (1-10 degrees centigrade)]. Pflugers Arch. 1969; 305(1):1-8. DOI: 10.1007/BF00586391. View

Nakajima Y, Endo M . Release of calcium induced by 'depolarisation' of the sarcoplasmic reticulum membrane. Nat New Biol. 1973; 246(155):216-8. DOI: 10.1038/newbio246216a0. View

Bloom S . Spontaneous rhythmic contraction of separated heart muscle cells. Science. 1970; 167(3926):1727-9. DOI: 10.1126/science.167.3926.1727. View

Bloom S . Phylogenetic differences in spontaneous contractility of isolated heart muscle cells. Comp Biochem Physiol. 1970; 37(1):127-9. DOI: 10.1016/0010-406x(70)90967-9. View

Reuter H, Seitz N . The dependence of calcium efflux from cardiac muscle on temperature and external ion composition. J Physiol. 1968; 195(2):451-70. PMC: 1351672. DOI: 10.1113/jphysiol.1968.sp008467. View

Fabiato A, Fabiato F . Excitation-contraction coupling of isolated cardiac fibers with disrupted or closed sarcolemmas. Calcium-dependent cyclic and tonic contractions. Circ Res. 1972; 31(3):293-307. DOI: 10.1161/01.res.31.3.293. View

Jensen R, KATZUNG B . Simultaneously recorded oscillations in membrane potential and isometric contractile force from cardiac muscle. Nature. 1968; 217(5132):961-3. DOI: 10.1038/217961a0. View

10.

GLITSCH H, Reuter H, Scholz H . The effect of the internal sodium concentration on calcium fluxes in isolated guinea-pig auricles. J Physiol. 1970; 209(1):25-43. PMC: 1396029. DOI: 10.1113/jphysiol.1970.sp009153. View

11.

Miller D . Proceedings: Evidence for calcium-induced release in intact depolarized frog heart muscle. J Physiol. 1974; 242(2):93P-95P. View

12.

Bloom S . Requirements for spontaneous contractility in isolated adult mammalian heart muscle cells. Exp Cell Res. 1971; 69(1):17-24. DOI: 10.1016/0014-4827(71)90304-1. View

13.

Langer G . Heart: excitation-contraction coupling. Annu Rev Physiol. 1973; 35:55-86. DOI: 10.1146/annurev.ph.35.030173.000415. View

14.

LUTTGAU H, NIEDERGERKE R . The antagonism between Ca and Na ions on the frog's heart. J Physiol. 1958; 143(3):486-505. PMC: 1356728. DOI: 10.1113/jphysiol.1958.sp006073. View

15.

Steiger G . Stretch activation and myogenic oscillation of isolated contractile structures of heart muscle. Pflugers Arch. 1971; 330(4):347-61. DOI: 10.1007/BF00588586. View

16.

Bloom S, BRADY A, Langer G . Calcium metabolism and active tension in mechanically disaggregated heart muscle. J Mol Cell Cardiol. 1974; 6(2):137-47. DOI: 10.1016/0022-2828(74)90017-0. View

17.

Reuter H . Divalent cations as charge carriers in excitable membranes. Prog Biophys Mol Biol. 1973; 26:1-43. DOI: 10.1016/0079-6107(73)90016-3. View

18.

Scholz H . [On the effect of calcium and sodium on the potassium contracture of isolated guinea pig auricles]. Pflugers Arch. 1969; 308(4):315-32. DOI: 10.1007/BF00587183. View

19.

Reuter H . Exchange of calcium ions in the mammalian myocardium. Mechanisms and physiological significance. Circ Res. 1974; 34(5):599-605. DOI: 10.1161/01.res.34.5.599. View

20.

Ford L, PODOLSKY R . Calcium uptake and force development by skinned muscle fibres in EGTA buffered solutions. J Physiol. 1972; 223(1):1-19. PMC: 1331429. DOI: 10.1113/jphysiol.1972.sp009830. View