Glycoprotein K Specified by Herpes Simplex Virus Type 1 is Expressed on Virions As a Golgi Complex-dependent Glycosylated Species and Functions in Virion Entry

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2001 Nov 17

PMID 11711633

Citations 38

Authors

T P Foster

G V RYBACHUK

K G Kousoulas

Affiliations

Soon will be listed here.

Abstract

To facilitate detection of glycoprotein K (gK) specified by herpes simplex virus, a 12-amino-acid epitope tag was inserted within gK domain III. Recombinant virus gKprotC-DIII, expressing the tagged gK, was isolated. This virus formed wild-type plaques and replicated as efficiently as the wild-type KOS virus in Vero cells. Anti-protein C MAb detected high-mannose and Golgi complex-dependent glycosylated gK within cells as well as on purified virions. The gK-null virus DeltagK (gK(-/-)) entered Vero cells substantially more slowly than the wild-type KOS (gK(+/+)), while DeltagK virus grown in complementing VK302 cells (gK(-/+)) entered with entry kinetics similar to those of the KOS virus.

Citing Articles

Herpes Simplex Virus-1 Induced Serotonin-Associated Metabolic Pathways Correlate With Severity of Virus- and Inflammation-Associated Ocular Disease.

Battaglia D, Sanchez-Pino M, Nichols C, Foster T Front Microbiol. 2022; 13:859866.

PMID: 35391733 PMC: 8982329. DOI: 10.3389/fmicb.2022.859866.

A novel H129-based anterograde monosynaptic tracer exhibits features of strong labeling intensity, high tracing efficiency, and reduced retrograde labeling.

Yang H, Xiong F, Qin H, Yu Q, Sun J, Zhao H Mol Neurodegener. 2022; 17(1):6.

PMID: 35012591 PMC: 8744342. DOI: 10.1186/s13024-021-00508-6.

Two Sides to Every Story: Herpes Simplex Type-1 Viral Glycoproteins gB, gD, gH/gL, gK, and Cellular Receptors Function as Key Players in Membrane Fusion.

Jambunathan N, Clark C, Musarrat F, Chouljenko V, Rudd J, Kousoulas K Viruses. 2021; 13(9).

PMID: 34578430 PMC: 8472851. DOI: 10.3390/v13091849.

Contributions of the Four Essential Entry Glycoproteins to HSV-1 Tropism and the Selection of Entry Routes.

Hilterbrand A, Daly R, Heldwein E mBio. 2021; 12(2).

PMID: 33653890 PMC: 8092210. DOI: 10.1128/mBio.00143-21.

"Non-Essential" Proteins of HSV-1 with Essential Roles In Vivo: A Comprehensive Review.

Dogrammatzis C, Waisner H, Kalamvoki M Viruses. 2020; 13(1).

PMID: 33374862 PMC: 7824580. DOI: 10.3390/v13010017.

References

Sanders P, Wilkie N, Davison A . Thymidine kinase deletion mutants of herpes simplex virus type 1. J Gen Virol. 1982; 63(2):277-95. DOI: 10.1099/0022-1317-63-2-277. View

Foster T, Kousoulas K . Genetic analysis of the role of herpes simplex virus type 1 glycoprotein K in infectious virus production and egress. J Virol. 1999; 73(10):8457-68. PMC: 112865. DOI: 10.1128/JVI.73.10.8457-8468.1999. View

Bond V, Person S . Fine structure physical map locations of alterations that affect cell fusion in herpes simplex virus type 1. Virology. 1984; 132(2):368-76. DOI: 10.1016/0042-6822(84)90042-4. View

Lee G, Shapira S, Spear P . Fine mapping of mutations in the fusion-inducing MP strain of herpes simplex virus type 1. Virology. 1984; 136(1):100-9. DOI: 10.1016/0042-6822(84)90251-4. View

Bzik D, Fox B, DeLuca N, Person S . Nucleotide sequence of a region of the herpes simplex virus type 1 gB glycoprotein gene: mutations affecting rate of virus entry and cell fusion. Virology. 1984; 137(1):185-90. DOI: 10.1016/0042-6822(84)90022-9. View

Pellett P, Kousoulas K, Pereira L, Roizman B . Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants. J Virol. 1985; 53(1):243-53. PMC: 255021. DOI: 10.1128/JVI.53.1.243-253.1985. View

DebRoy C, Pederson N, Person S . Nucleotide sequence of a herpes simplex virus type 1 gene that causes cell fusion. Virology. 1985; 145(1):36-48. DOI: 10.1016/0042-6822(85)90199-0. View

Spear P . The single base pair substitution responsible for the Syn phenotype of herpes simplex virus type 1, strain MP. Virology. 1987; 157(1):67-74. DOI: 10.1016/0042-6822(87)90314-x. View

McGeoch D, Dalrymple M, Davison A, Dolan A, Frame M, McNab D . The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. J Gen Virol. 1988; 69 ( Pt 7):1531-74. DOI: 10.1099/0022-1317-69-7-1531. View

10.

Jacobson J, Martin S, Coen D . A conserved open reading frame that overlaps the herpes simplex virus thymidine kinase gene is important for viral growth in cell culture. J Virol. 1989; 63(4):1839-43. PMC: 248470. DOI: 10.1128/JVI.63.4.1839-1843.1989. View

11.

Sodora D, Cohen G, Eisenberg R . Influence of asparagine-linked oligosaccharides on antigenicity, processing, and cell surface expression of herpes simplex virus type 1 glycoprotein D. J Virol. 1989; 63(12):5184-93. PMC: 251182. DOI: 10.1128/JVI.63.12.5184-5193.1989. View

12.

Rice S, Knipe D . Genetic evidence for two distinct transactivation functions of the herpes simplex virus alpha protein ICP27. J Virol. 1990; 64(4):1704-15. PMC: 249308. DOI: 10.1128/JVI.64.4.1704-1715.1990. View

13.

Maclean C, Efstathiou S, Elliott M, Jamieson F, McGeoch D . Investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins. J Gen Virol. 1991; 72 ( Pt 4):897-906. DOI: 10.1099/0022-1317-72-4-897. View

14.

Mettenleiter T . Aujeszky's disease (pseudorabies) virus: the virus and molecular pathogenesis--state of the art, June 1999. Vet Res. 2000; 31(1):99-115. DOI: 10.1051/vetres:2000110. View

15.

Skepper J, Whiteley A, Browne H, Minson A . Herpes simplex virus nucleocapsids mature to progeny virions by an envelopment --> deenvelopment --> reenvelopment pathway. J Virol. 2001; 75(12):5697-702. PMC: 114284. DOI: 10.1128/JVI.75.12.5697-5702.2001. View

16.

Foster T, Melancon J, Kousoulas K . An alpha-helical domain within the carboxyl terminus of herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) is associated with cell fusion and resistance to heparin inhibition of cell fusion. Virology. 2001; 287(1):18-29. DOI: 10.1006/viro.2001.1004. View

17.

Ruyechan W, Morse L, Knipe D, Roizman B . Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells. J Virol. 1979; 29(2):677-97. PMC: 353200. DOI: 10.1128/JVI.29.2.677-697.1979. View

18.

Read G, Person S, Keller P . Genetic studies of cell fusion induced by herpes simplex virus type 1. J Virol. 1980; 35(1):105-13. PMC: 288786. DOI: 10.1128/JVI.35.1.105-113.1980. View

19.

Bond V, Person S, Warner S . The isolation and characterization of mutants of herpes simplex virus type 1 that induce cell fusion. J Gen Virol. 1982; 61 (Pt 2):245-54. DOI: 10.1099/0022-1317-61-2-245. View

20.

Baines J, Ward P, Roizman B . The UL20 gene of herpes simplex virus 1 encodes a function necessary for viral egress. J Virol. 1991; 65(12):6414-24. PMC: 250678. DOI: 10.1128/JVI.65.12.6414-6424.1991. View