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Interferon-gamma Secretion of Peripheral Blood CD8+ T Lymphocytes in Patients with Bronchial Asthma: in Vitro Stimulus Determines Cytokine Production

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Date 2001 Nov 13
PMID 11703361
Citations 7
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Abstract

It has been postulated that T lymphocytes orchestrate the chronic inflammation in bronchial asthma. In animal models, infiltration of CD8+ T lymphocytes into the bronchial mucosa prevented bronchial hyperresponsiveness and decreased early and late phase reaction. IFN-gamma antagonizes IL-4-dependent IgE production as well as IL-5-induced proliferation and activation of eosinophils. We therefore investigated the secretion of IFN-gamma of isolated CD8+ T lymphocytes from peripheral blood of patients with allergic asthma (n = 6) and from healthy controls (n = 7) in vitro. In this setting we compared the effect of stimulation with anti-CD3 antibodies with that of phorbol myristate acetate (PMA) and calcium-ionophore. As expected, CD8+ T lymphocytes from peripheral blood of healthy volunteers produced significantly more IFN-gamma in the presence of PMA and calcium-ionophore than after stimulation with anti-CD3 antibodies. However, in subjects with allergic asthma, IFN-gamma secretion of CD8+ T cells was significantly higher when incubated with anti-CD3 antibodies than after activation with PMA and calcium-ionophore. While IFN-gamma secretion of CD8+ T lymphocytes of patients with allergic asthma was lower than that of healthy controls in the presence of PMA/calcium-ionophore, it was significantly elevated when compared with normal controls after stimulation with anti-CD3 antibodies. Thus, potent activators of cytokine secretion, such as PMA and calcium-ionophore, induce a cytokine profile different from that induced by weaker stimulants, such as anti-CD3 antibodies. These findings have implications for further studies investigating cytokine production of inflammatory cells in vitro.

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References
1.
Gonzalez M, Diaz P, Galleguillos F, Ancic P, Cromwell O, Kay A . Allergen-induced recruitment of bronchoalveolar helper (OKT4) and suppressor (OKT8) T-cells in asthma. Relative increases in OKT8 cells in single early responders compared with those in late-phase responders. Am Rev Respir Dis. 1987; 136(3):600-4. DOI: 10.1164/ajrccm/136.3.600. View

2.
Luttmann W, Herzog V, Virchow Jr J, Matthys H, Thierauch K, Kroegel C . Prostacyclin modulates granulocyte/macrophage colony-stimulating factor release by human blood mononuclear cells. Pulm Pharmacol. 1996; 9(1):43-8. DOI: 10.1006/pulp.1996.0005. View

3.
Romagnani S, Maggi E, Del Prete G, Parronchi P, Macchia D, Tiri A . Role of interleukin 4 and gamma interferon in the regulation of human IgE synthesis: possible alterations in atopic patients. Int Arch Allergy Appl Immunol. 1989; 88(1-2):111-3. DOI: 10.1159/000234758. View

4.
Miltenyi S, Muller W, Weichel W, Radbruch A . High gradient magnetic cell separation with MACS. Cytometry. 1990; 11(2):231-8. DOI: 10.1002/cyto.990110203. View

5.
Borish L, Rosenwasser L . TH1/TH2 lymphocytes: doubt some more. J Allergy Clin Immunol. 1997; 99(2):161-4. DOI: 10.1016/s0091-6749(97)70090-3. View