[Riboflavin Auxotrophs of Escherichia Coli]

Overview

Journal Genetika

Publisher Izdatelstvo Nauka

Specialty Genetics

Date 1979 Nov 1

PMID 116904

Citations 9

Authors

S V Bandrin

M Iu Beburov

P M Rabinovich

A I Stepanov

Affiliations

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Abstract

Escherichia coli riboflavin auxotrophs having a different level of riboflavin requirement were isolated. This auxotrophic mutations are located near cysB93 and trpA62 markers. The complementary effect of Bacillus subtilis riboflavin operon linked with pPR1 hybrid plasmid with rib8-1 and rib1-1 mutations was obtained.

Citing Articles

A set of engineered Escherichia coli expression strains for selective isotope and reactivity labeling of amino acid side chains and flavin cofactors.

Mehlhorn J, Steinocher H, Beck S, Kennis J, Hegemann P, Mathes T PLoS One. 2013; 8(11):e79006.

PMID: 24223875 PMC: 3815312. DOI: 10.1371/journal.pone.0079006.

RibM from Streptomyces davawensis is a riboflavin/roseoflavin transporter and may be useful for the optimization of riboflavin production strains.

Hemberger S, Pedrolli D, Stolz J, Vogl C, Lehmann M, Mack M BMC Biotechnol. 2011; 11:119.

PMID: 22136195 PMC: 3239331. DOI: 10.1186/1472-6750-11-119.

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.

Abbas C, Sibirny A Microbiol Mol Biol Rev. 2011; 75(2):321-60.

PMID: 21646432 PMC: 3122625. DOI: 10.1128/MMBR.00030-10.

(15)N{(31)P} REDOR NMR studies of the binding of phosphonate reaction intermediate analogues to Saccharomyces cerevisiae lumazine synthase.

Yu T, OConnor R, Sivertsen A, Chiauzzi C, Poliks B, Fischer M Biochemistry. 2009; 47(52):13942-51.

PMID: 19117095 PMC: 2630582. DOI: 10.1021/bi8015789.

The bifunctional flavokinase/flavin adenine dinucleotide synthetase from Streptomyces davawensis produces inactive flavin cofactors and is not involved in resistance to the antibiotic roseoflavin.

Grill S, Busenbender S, Pfeiffer M, Kohler U, Mack M J Bacteriol. 2007; 190(5):1546-53.

PMID: 18156273 PMC: 2258686. DOI: 10.1128/JB.01586-07.