Ribosomal and Informational Ribonucleoprotein Complexes of Animal Cells. Study on Rat Liver Ribonucleic Acids As Constituents of Ribonucleoprotein Complexes by Chromatography on Nucleoprotein-celite Columns

Overview

Journal Biochem J

Specialty Biochemistry

Date 1975 Jun 1

PMID 1167154

Citations 1

Authors

A V Lichtenstein

R P Alechina

V S SHAPOT

Affiliations

Soon will be listed here.

Abstract

A novel method of RNA fractionation has been developed. Nuclear and cytoplasmic rat liver RNA species were fractionated as constituents of corresponding ribonucleoprotein particles, which were previously adsorbed on a Celite-column by their protein component. The fractionation is based on a dissociation of the particles (linear concentration gradient of LiCl and urea with subsequent temperature gradient), which results in a gradual release of the RNA molecules from ribonucleoprotein complexes. Thus the fractionation is in accordance with the tightness of the RNA-protein bonds. A gradient elution of RNA from a nucleoprotein-Celite column permitted fractionation of both ribosomal and rapidly labelled non-ribosomal RNA. The latter, both nuclear and cytoplasmic, could be separated by chromatography on nucleoprotein-Celite columns into two main fractions (components I and V). In cytoplasmic RNA components I and V presumably correspond to mlRNA (messenger-like RNA of free cytoplasmic particles) and mRNA (template RNA associated with ribosomes) respectively.

Citing Articles

Rearrangements of DNA-protein interactions in animal cells coupled with cellular growth-quiescence transitions.

Lichtenstein A, Sjakste N, Zaboykin M, SHAPOT V Nucleic Acids Res. 1982; 10(3):1127-45.

PMID: 7063419 PMC: 326227. DOI: 10.1093/nar/10.3.1127.

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