Enrichment of Memory Cells Carrying Receptors for a Protein Antigen (HSA). II. Improved Enrichment Technique, Using BSA Density Gradient Separation

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1975 Sep 1

PMID 1165105

Citations 1

Authors

R Tanenbaum

D Sulitzeanu

Affiliations

Soon will be listed here.

Abstract

Mice primed to human serum albumin (HSA) by means of an HSA--sheep red cell conjugate, developed a high level of memory to HSA, with little concomitant antibody production ('pure priming'). The proportion of HSA-specific antigen-binding cells was determined in the spleens of the primed mice by means of a rosette technique, using an HSA-donkey red cell conjugate. The specificity of the rosette-forming cells (RFC) was confirmed by the ability of soluble HSA to inhibit rosette formation. Highly enriched rosette suspensions (up to 40-fold enrichment) were prepared by bovine serum albumin density gradient centrifugation. The enriched suspensions could transfer adoptively memory to HSA, whereas the rosette-depleted fractions were totally inactive. It was thus confirmed that memory cells are included in the antigen-binding cells. A detectable level of memory could be transferred with 3000 rosettes. RFC similarly isolated from spleens of non-primed mice could also transfer a low level of responsiveness to HSA, but only when large numbers (70,000) were injected, together with primed, RFC-depleted spleen cells.

Citing Articles

High gradient magnetic separation of rosette-forming cells.

Owen C, Moore E Cell Biophys. 1981; 3(2):141-53.

PMID: 6168377 DOI: 10.1007/BF02788130.

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