Characterization of Schizosaccharomyces Pombe Mcm7(+) and Cdc23(+) (MCM10) and Interactions with Replication Checkpoints

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2001 Oct 19

PMID 11606526

Citations 24

Authors

D T Liang

S L Forsburg

Affiliations

Soon will be listed here.

Abstract

MCM proteins are required for the proper regulation of DNA replication. We cloned fission yeast mcm7(+) and showed it is essential for viability; spores lacking mcm7(+) begin S phase later than wild-type cells and arrest with an apparent 2C DNA content. We isolated a novel temperature-sensitive allele, mcm7-98, and also characterized two temperature-sensitive alleles of the fission yeast homolog of MCM10, cdc23(+). mcm7-98 and both cdc23ts alleles arrest with damaged chromosomes and an S phase delay. We find that mcm7-98 is synthetically lethal with the other mcmts mutants but does not interact genetically with either cdc23ts allele. However, cdc23-M36 interacts with mcm4ts. Unlike other mcm mutants or cdc23, mcm7-98 is synthetically lethal with checkpoint mutants Deltacds1, Deltachk1, or Deltarad3, suggesting chromosomal defects even at permissive temperature. Mcm7p is a nuclear protein throughout the cell cycle, and its localization is dependent on the other MCM proteins. Our data suggest that the Mcm3p-Mcm5p dimer interacts with the Mcm4p-Mcm6p-Mcm7p core complex through Mcm7p.

Citing Articles

Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation.

Vo N, Suong D, Yoshino N, Yoshida H, Cotterill S, Yamaguchi M Nucleic Acids Res. 2017; 45(3):1233-1254.

PMID: 28180289 PMC: 5388399. DOI: 10.1093/nar/gkw1174.

Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Ranatunga N, Forsburg S G3 (Bethesda). 2016; 6(10):3049-3063.

PMID: 27473316 PMC: 5068930. DOI: 10.1534/g3.116.033571.

Replication stress in early S phase generates apparent micronuclei and chromosome rearrangement in fission yeast.

Sabatinos S, Ranatunga N, Yuan J, Green M, Forsburg S Mol Biol Cell. 2015; 26(19):3439-50.

PMID: 26246602 PMC: 4591689. DOI: 10.1091/mbc.E15-05-0318.

Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L, Forsburg S G3 (Bethesda). 2014; 4(8):1373-84.

PMID: 24875629 PMC: 4132169. DOI: 10.1534/g3.114.011346.

Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7.

Xu M, Chang Y, Chen X BMC Biochem. 2013; 14:5.

PMID: 23444842 PMC: 3605359. DOI: 10.1186/1471-2091-14-5.

References

Fenech M, Carr A, Murray J, Watts F, Lehmann A . Cloning and characterization of the rad4 gene of Schizosaccharomyces pombe; a gene showing short regions of sequence similarity to the human XRCC1 gene. Nucleic Acids Res. 1991; 19(24):6737-41. PMC: 329303. DOI: 10.1093/nar/19.24.6737. View

Sato M, Gotow T, You Z, Uchiyama Y, Yabuta N, Nojima H . Electron microscopic observation and single-stranded DNA binding activity of the Mcm4,6,7 complex. J Mol Biol. 2000; 300(3):421-31. DOI: 10.1006/jmbi.2000.3865. View

Ohi R, Feoktistova A, Gould K . Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe. Gene. 1996; 174(2):315-8. DOI: 10.1016/0378-1119(96)00085-6. View

Oconnell M, Walworth N, Carr A . The G2-phase DNA-damage checkpoint. Trends Cell Biol. 2000; 10(7):296-303. DOI: 10.1016/s0962-8924(00)01773-6. View

Aparicio O, Weinstein D, Bell S . Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell. 1997; 91(1):59-69. DOI: 10.1016/s0092-8674(01)80009-x. View

Tye B . MCM proteins in DNA replication. Annu Rev Biochem. 2000; 68:649-86. DOI: 10.1146/annurev.biochem.68.1.649. View

Maine G, Sinha P, Tye B . Mutants of S. cerevisiae defective in the maintenance of minichromosomes. Genetics. 1984; 106(3):365-85. PMC: 1224244. DOI: 10.1093/genetics/106.3.365. View

Steyerberg E, Kievit J, de Mol van Otterloo J, van Bockel J, Eijkemans M, Habbema J . Perioperative mortality of elective abdominal aortic aneurysm surgery. A clinical prediction rule based on literature and individual patient data. Arch Intern Med. 1995; 155(18):1998-2004. View

Maiorano D, Van Assendelft G, Kearsey S . Fission yeast cdc21, a member of the MCM protein family, is required for onset of S phase and is located in the nucleus throughout the cell cycle. EMBO J. 1996; 15(4):861-72. PMC: 450284. View

10.

Forsburg S, Sherman D . General purpose tagging vectors for fission yeast. Gene. 1997; 191(2):191-5. DOI: 10.1016/s0378-1119(97)00058-9. View

11.

Dutta A, Bell S . Initiation of DNA replication in eukaryotic cells. Annu Rev Cell Dev Biol. 1997; 13:293-332. DOI: 10.1146/annurev.cellbio.13.1.293. View

12.

Nasmyth K, Nurse P . Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet. 1981; 182(1):119-24. DOI: 10.1007/BF00422777. View

13.

Izumi M, Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon K . The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase. Nucleic Acids Res. 2000; 28(23):4769-77. PMC: 115166. DOI: 10.1093/nar/28.23.4769. View

14.

Boddy M, Furnari B, Mondesert O, Russell P . Replication checkpoint enforced by kinases Cds1 and Chk1. Science. 1998; 280(5365):909-12. DOI: 10.1126/science.280.5365.909. View

15.

Sherman D, Forsburg S . Schizosaccharomyces pombe Mcm3p, an essential nuclear protein, associates tightly with Nda4p (Mcm5p). Nucleic Acids Res. 1998; 26(17):3955-60. PMC: 147805. DOI: 10.1093/nar/26.17.3955. View

16.

Romanowski P, Madine M, Laskey R . XMCM7, a novel member of the Xenopus MCM family, interacts with XMCM3 and colocalizes with it throughout replication. Proc Natl Acad Sci U S A. 1996; 93(19):10189-94. PMC: 38359. DOI: 10.1073/pnas.93.19.10189. View

17.

Barbet N, Muriel W, Carr A . Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene. 1992; 114(1):59-66. DOI: 10.1016/0378-1119(92)90707-v. View

18.

Araki H, Leem S, Phongdara A, Sugino A . Dpb11, which interacts with DNA polymerase II(epsilon) in Saccharomyces cerevisiae, has a dual role in S-phase progression and at a cell cycle checkpoint. Proc Natl Acad Sci U S A. 1995; 92(25):11791-5. PMC: 40488. DOI: 10.1073/pnas.92.25.11791. View

19.

Forsburg S, Sherman D, Ottilie S, Yasuda J, Hodson J . Mutational analysis of Cdc19p, a Schizosaccharomyces pombe MCM protein. Genetics. 1998; 147(3):1025-41. PMC: 1208231. DOI: 10.1093/genetics/147.3.1025. View

20.

Saka Y, Fantes P, Sutani T, McInerny C, Creanor J, Yanagida M . Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. EMBO J. 1994; 13(22):5319-29. PMC: 395488. DOI: 10.1002/j.1460-2075.1994.tb06866.x. View