PIRA PCR Designer for Restriction Analysis of Single Nucleotide Polymorphisms

Overview

Journal Bioinformatics

Publisher Oxford University Press

Specialty Biology

Date 2001 Oct 9

PMID 11590100

Citations 29

Authors

X Ke

A Collins

S Ye

Affiliations

Soon will be listed here.

Abstract

Unlabelled: Primer-introduced restriction analysis (PIRA-PCR) is widely used to detect Single Nucleotide Polymorphisms (SNPs). To create artificial Restriction Fragment Length Polymorphism (RFLP), a mismatch is usually introduced near the end of the primer that is close to the mutation of interest. We describe in this report a www-based computer program that screens for the suitable mismatches, designs the primers, lists the appropriate restriction enzymes and other related information.

Availability: The computer program, with related descriptions, is available at http://cedar.genetics.soton.ac.uk/public_html/primer2.html.

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