Molecular Cloning of a CDNA Encoding Mouse DNA Helicase B, Which Has Homology to Escherichia Coli RecD Protein, and Identification of a Mutation in the DNA Helicase B from TsFT848 Temperature-sensitive DNA Replication Mutant Cells

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2001 Sep 15

PMID 11557815

Citations 10

Authors

S Tada

T Kobayashi

A Omori

Y Kusa

N Okumura

H Kodaira

Y Ishimi

M Seki

T Enomoto

Affiliations

Soon will be listed here.

Abstract

DNA helicase B is a major DNA helicase in mouse FM3A cells. A temperature-sensitive mutant defective in DNA replication, tsFT848, isolated from FM3A cells, has a heat-labile DNA helicase B. In this study, we purified DNA helicase B from mouse FM3A cells and determined partial amino acid sequences of the purified protein. By using a DNA probe synthesized according to one of the partial amino acid sequences, a cDNA was isolated, which encoded a 121.5 kDa protein containing seven conserved motifs for DNA/RNA helicase superfamily members. A database search revealed similarity between DNA helicase B and the alpha subunit of exodeoxyribonuclease V of a number of prokaryotes including Escherichia coli RecD protein, but no homologous protein was found in yeast. The cDNA encoding DNA helicase B from tsFT848 was sequenced and a mutation was found between DNA/RNA helicase motifs IV and V.

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