Impact of the Six Nucleotides Downstream of the Stop Codon on Translation Termination

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2001 Aug 25

PMID 11520858

Citations 122

Authors

O Namy

I Hatin

J P Rousset

Affiliations

Soon will be listed here.

Abstract

The efficiency of translation termination is influenced by local contexts surrounding stop codons. In Saccharomyces cerevisiae, upstream and downstream sequences act synergistically to influence the translation termination efficiency. By analysing derivatives of a leaky stop codon context, we initially demonstrated that at least six nucleotides after the stop codon are a key determinant of readthrough efficiency in S. cerevisiae. We then developed a combinatorial-based strategy to identify poor 3' termination contexts. By screening a degenerate oligonucleotide library, we identified a consensus sequence -CA(A/G)N(U/C/G)A-, which promotes >5% readthrough efficiency when located downstream of a UAG stop codon. Potential base pairing between this stimulatory motif and regions close to helix 18 and 44 of the 18S rRNA provides a model for the effect of the 3' stop codon context on translation termination.

Citing Articles

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Regulatory significance of terminator: A systematic approach for dissecting terminator-mediated enhancement of upstream mRNA stability.

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The ABCF ATPase New1 resolves translation termination defects associated with specific tRNAArg and tRNALys isoacceptors in the P site.

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