Autophosphorylation of Archaeal Cdc6 Homologues is Regulated by DNA

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2001 Aug 22

PMID 11514535

Citations 23

Authors

B Grabowski

Z Kelman

Affiliations

Soon will be listed here.

Abstract

The initiator protein Cdc6 (Cdc18 in fission yeast) plays an essential role in the initiation of eukaryotic DNA replication. In yeast the protein is expressed before initiation of DNA replication and is thought to be essential for loading of the helicase onto origin DNA. The biochemical properties of the protein, however, are largely unknown. Using three archaeal homologues of Cdc6, it was found that the proteins are autophosphorylated on Ser residues. The winged-helix domain at the C terminus of Cdc6 interacts with DNA, which apparently regulates the autophosphorylation reaction. Yeast Cdc18 was also found to autophosphorylate, suggesting that this function of Cdc6 may play a widely conserved and essential role in replication initiation.

Citing Articles

The Archaeal Cell Cycle.

Cezanne A, Foo S, Kuo Y, Baum B Annu Rev Cell Dev Biol. 2024; 40(1):1-23.

PMID: 38748857 PMC: 7617429. DOI: 10.1146/annurev-cellbio-111822-120242.

Dissection of Functional Domains of Orc1-2, the Archaeal Global DNA Damage-Responsive Regulator.

Liu X, Sun M, Xu R, Shen Y, Huang Q, Feng X Int J Mol Sci. 2022; 23(23).

PMID: 36498936 PMC: 9738581. DOI: 10.3390/ijms232314609.

Post-Translational Modifications Aid Archaeal Survival.

Gong P, Lei P, Wang S, Zeng A, Lou H Biomolecules. 2020; 10(4).

PMID: 32290118 PMC: 7226565. DOI: 10.3390/biom10040584.

Protein phosphorylation and its role in archaeal signal transduction.

Esser D, Hoffmann L, Pham T, Brasen C, Qiu W, Wright P FEMS Microbiol Rev. 2016; 40(5):625-47.

PMID: 27476079 PMC: 5007285. DOI: 10.1093/femsre/fuw020.

The Mcm complex: unwinding the mechanism of a replicative helicase.

Bochman M, Schwacha A Microbiol Mol Biol Rev. 2009; 73(4):652-83.

PMID: 19946136 PMC: 2786579. DOI: 10.1128/MMBR.00019-09.

References

Shechter D, YING C, Gautier J . The intrinsic DNA helicase activity of Methanobacterium thermoautotrophicum delta H minichromosome maintenance protein. J Biol Chem. 2000; 275(20):15049-59. DOI: 10.1074/jbc.M000398200. View

Lee D, Bell S . ATPase switches controlling DNA replication initiation. Curr Opin Cell Biol. 2000; 12(3):280-5. DOI: 10.1016/s0955-0674(00)00089-2. View

Tye B . MCM proteins in DNA replication. Annu Rev Biochem. 2000; 68:649-86. DOI: 10.1146/annurev.biochem.68.1.649. View

Mizushima T, Takahashi N, Stillman B . Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro. Genes Dev. 2000; 14(13):1631-41. PMC: 316732. View

Kelly T, Brown G . Regulation of chromosome replication. Annu Rev Biochem. 2000; 69:829-80. DOI: 10.1146/annurev.biochem.69.1.829. View

Davey M, ODonnell M . Mechanisms of DNA replication. Curr Opin Chem Biol. 2000; 4(5):581-6. DOI: 10.1016/s1367-5931(00)00134-4. View

Liu J, Smith C, DeRyckere D, DeAngelis K, Martin G, Berger J . Structure and function of Cdc6/Cdc18: implications for origin recognition and checkpoint control. Mol Cell. 2000; 6(3):637-48. DOI: 10.1016/s1097-2765(00)00062-9. View

Kelman Z . DNA replication in the third domain (of life). Curr Protein Pept Sci. 2002; 1(2):139-54. DOI: 10.2174/1389203003381414. View

Zeikus J, WOLFE R . Methanobacterium thermoautotrophicus sp. n., an anaerobic, autotrophic, extreme thermophile. J Bacteriol. 1972; 109(2):707-15. PMC: 285196. DOI: 10.1128/jb.109.2.707-713.1972. View

10.

Zylicz M, Lebowitz J, McMacken R, Georgopoulos C . The dnaK protein of Escherichia coli possesses an ATPase and autophosphorylating activity and is essential in an in vitro DNA replication system. Proc Natl Acad Sci U S A. 1983; 80(21):6431-5. PMC: 390127. DOI: 10.1073/pnas.80.21.6431. View

11.

Walker J, Saraste M, Runswick M, Gay N . Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982; 1(8):945-51. PMC: 553140. DOI: 10.1002/j.1460-2075.1982.tb01276.x. View

12.

Story R, Steitz T . Structure of the recA protein-ADP complex. Nature. 1992; 355(6358):374-6. DOI: 10.1038/355374a0. View

13.

Diffley J, Cocker J, Dowell S, Rowley A . Two steps in the assembly of complexes at yeast replication origins in vivo. Cell. 1994; 78(2):303-16. DOI: 10.1016/0092-8674(94)90299-2. View

14.

Mahoney C, Nakanishi N, Ohashi M . Phosphoamino acid analysis by semidry electrophoresis on cellulose thin-layer plates using the Pharmacia/LKB Multiphor or Atto flatbed apparatus. Anal Biochem. 1996; 238(1):96-8. DOI: 10.1006/abio.1996.0258. View

15.

Subramanya H, BIRD L, Brannigan J, Wigley D . Crystal structure of a DExx box DNA helicase. Nature. 1996; 384(6607):379-83. DOI: 10.1038/384379a0. View

16.

DONOVAN S, Harwood J, Drury L, Diffley J . Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast. Proc Natl Acad Sci U S A. 1997; 94(11):5611-6. PMC: 20826. DOI: 10.1073/pnas.94.11.5611. View

17.

Tanaka T, Knapp D, Nasmyth K . Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell. 1997; 90(4):649-60. DOI: 10.1016/s0092-8674(00)80526-7. View

18.

Drury L, Perkins G, Diffley J . The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J. 1997; 16(19):5966-76. PMC: 1170227. DOI: 10.1093/emboj/16.19.5966. View

19.

Jallepalli P, Brown G, Muzi-Falconi M, Tien D, Kelly T . Regulation of the replication initiator protein p65cdc18 by CDK phosphorylation. Genes Dev. 1997; 11(21):2767-79. PMC: 316667. DOI: 10.1101/gad.11.21.2767. View

20.

Smith D, DeLoughery C, Lee H, Dubois J, Aldredge T, Bashirzadeh R . Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics. J Bacteriol. 1997; 179(22):7135-55. PMC: 179657. DOI: 10.1128/jb.179.22.7135-7155.1997. View