Different Effects of Lectins on the Ligand Binding of the NMDA Receptors and Sigma Sites in Rat Brain Hippocampus Synaptic Membranes

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2001 Aug 22

PMID 11513469

Citations 2

Authors

G G Machaidze

D Mikeladze

Affiliations

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Abstract

The effects of the lectins concanavalin A, WGA, ricin, abrin, and the mistletoe lectins from Viscum album MLI, MLII, and MLIII on the binding of ligands of the NMDA and sigma receptors in rat hippocampus synaptic plasma membranes were investigated. Binding of [3H]MK-801, [3H]glutamate, [3H]5,7-DCKA, and [3H]glycine to the membranes was decreased by 40-60% after addition of galactose-specific lectins (mistletoe lectins MLI, MLII, ricin, abrin) at concentrations of 0.01 mg/ml, but was not affected by the glucose- and mannose-specific lectin Con A, an acetylglucosamine-specific lectin WGA, or an acetylgalactosamine-specific lectin MLIII. The binding of [3H]SKF 10047 was decreased only in the presence of MLIII and did not change after addition of the other lectins. It is suggested that lectin-sensitive ligand binding sites of sigma- and NMDA receptors are located separately, and that the carbohydrate side chains of the sigma receptor do not participate in the modulation of the NMDA-receptor.

Citing Articles

Membrane Binding of Neuronal Calcium Sensor-1: Highly Specific Interaction with Phosphatidylinositol-3-Phosphate.

Baksheeva V, Nemashkalova E, Firsov A, Zalevsky A, Vladimirov V, Tikhomirova N Biomolecules. 2020; 10(2).

PMID: 31973069 PMC: 7072451. DOI: 10.3390/biom10020164.

ConBr, a lectin from Canavalia brasiliensis seeds, protects against quinolinic acid-induced seizures in mice.

Russi M, Vandresen-Filho S, Rieger D, Costa A, Lopes M, Cunha R Neurochem Res. 2011; 37(2):288-97.

PMID: 21948344 DOI: 10.1007/s11064-011-0608-x.

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