Effects of Gamma-irradiation on Biosynthesis of Different Types of Ribonucleic Acids in Normal and Regenerating Rat Liver

Overview

Journal Biochem J

Specialty Biochemistry

Date 1975 Jan 1

PMID 1147904

Authors

G G MARKOV

G N Dessev

G C. Russev

R G TSANEV

Affiliations

Soon will be listed here.

Abstract

1. The effect of gamma-irradiation (4000rd) on the synthesis of ribosomal (pre-rRNA) and heterogeneous nuclear RNA (pre-mRNA) in normal and in regenerating rat liver was studied by using 40 min labelling with [6(-14)C]orotic acid. 2. Partial hepatectomy caused a sharp transient increase in the specific radioactivity of the endogenous low-molecular-weight RNA precursors in the livers of both normal and irradiated rats. Irradiation of intact animals did not affect the pool. 3. Irradiation enhanced the synthesis of pre-rRNA for at least 12h. The synthesis of pre-mRNA was also enhanced, but only in the first 3h after irradiation. 4. Partial hepatectomy strongly stimulated the synthesis of both pre-rRNA and pre-mRNA. 5. The synthesis of pre-rRNA was enhanced also in regenerating liver of animals irradiated before or after the operation. The conclusion can be drawn that the early increase in the synthesis of ribosomal RNA is a non-specific cellular response to different injuring factors. 6. The only case where irradiation caused an early inhibition of RNA synthesis was that of pre-mRNA in regenerating liver. This supports the hypothesis that ionizing radiation does not suppress the transcription per se but affects the mechanisms of activation of new genes (cellular programming).

References

van Huystee R, JACHYMCZYK W, Tester C, Cherry J . X-irradiation effects on protein synthesis and synthesis of messenger ribonucleic acid from peanut cotyledons. J Biol Chem. 1968; 243(9):2315-20. View

Arion V, MANTIEVA V, Georgiev G . Metabolically stable and metabolically labile DNA-like RNA. Biochim Biophys Acta. 1967; 138(2):436-8. DOI: 10.1016/0005-2787(67)90506-0. View

SIMIC M, HUDNIK-PLEVNIK T, Kanazir D . Effect of x-irradiation on the synthesis and composition of pulse-labelled RNA of the liver of the rat foetus. Nature. 1967; 214(5087):489-90. DOI: 10.1038/214489a0. View

Church R, McCarthy B . Ribonucleic acid synthesis in regenerating and embryonic liver. I. The synthesis of new species of RNA during regeneration of mouse liver after partial hepatectomy. J Mol Biol. 1967; 23(3):459-75. DOI: 10.1016/s0022-2836(67)80118-9. View

KAFIANI K, TIMOFEEVA M, NEIFAKH A, Rachkus I, Melnikova N . [The effect of X-irradiation on the synthesis of messenger RNA at the early stages of loach embryogenesis]. Biokhimiia. 1966; 31(2):365-71. View

TSANEV R, MARKOV G, Dessev G . Incorporation of labelled precursors into the electrophoretic fractions of rat-liver ribonucleic acid. Biochem J. 1966; 100(1):204-10. PMC: 1265111. DOI: 10.1042/bj1000204. View

Uchiyama T, Fausto N, VAN LANCKER J . Molecular mechanisms of liver regeneration. V. The effect of x-radiation on the incorporation of 14C-orotic acid rapidly labeled nuclear ribonucleic acid. J Biol Chem. 1966; 241(4):991-6. View

BRESNICK E . EARLY CHANGES IN PYRIMIDINE BIOSYNTHESIS AFTER PARTIAL HEPATECTOMY. J Biol Chem. 1965; 240:2550-6. View

Ames B, HORECKER B . The biosynthesis of histidine: imidazoleacetol phosphate transaminase. J Biol Chem. 1956; 220(1):113-28. View

10.

BUCHER N . REGENERATION OF MAMMALIAN LIVER. Int Rev Cytol. 1963; 15:245-300. DOI: 10.1016/s0074-7696(08)61119-5. View

11.

MARKOV G . STUDIES ON THE ACQUIRED RADIORESISTANCE OF THE EHRLICH ASCITES TUMOR. Acta Unio Int Contra Cancrum. 1964; 20:1352-4. View

12.

KELLY L . Radiosensitivity of biochemical processes. Brookhaven Symp Biol. 1961; 14:32-52. View

13.

Georgiev G, MANTIEVA V . The isolation of DNA-like RNA and ribosomal RNA from the nucleolo-chromosomal apparatus of mammalian cells. Biochim Biophys Acta. 1962; 61:153-4. DOI: 10.1016/0926-6550(62)90046-4. View

14.

ZILVERSMIT D . The design and analysis of isotope experiments. Am J Med. 1960; 29:832-48. DOI: 10.1016/0002-9343(60)90117-0. View

15.

Whitmore G, Stanners C, Till J, Gulyas S . Nucleic acid synthesis and the division cycle in x-irradiated L-strain mouse cells. Biochim Biophys Acta. 1961; 47:66-77. DOI: 10.1016/0006-3002(61)90830-7. View

16.

ALBERT M, BUCHER N . Latent injury and repair in rat liver induced to regenerate at intervals after x-radiation. Cancer Res. 1960; 20:1514-22. View

17.

HURLBERT R, POTTER V . Nucleotide metabolism. I. The conversion of orotic acid-6-C14 to uridine nucleotides. J Biol Chem. 1954; 209(1):1-21. View

18.

Klein G, FORSSBERG A . Studies on the effect of x-rays on the biochemistry and cellular composition of ascites tumors. I. Effect on growth rate, cell volume, nucleic acid and nitrogen content in the Ehrlich ascites tumor. Exp Cell Res. 1954; 6(1):211-20. DOI: 10.1016/0014-4827(54)90163-9. View

19.

Djordjevic B, Kim J, Kim S . Modification of radiation response in synchronized HeLa cells by metabolic inhibitors. I. Effect of an inhibitor of RNA-synthesis, puromycin aminonucleoside. Int J Radiat Biol Relat Stud Phys Chem Med. 1968; 14(1):1-7. DOI: 10.1080/09553006814550721. View

20.

Tasheva B, Markov G, TSANEV R . Effect of irradiation on amino acid in vitro incorporation in the course of liver regeneration. Z Naturforsch C. 1973; 28(5):313-5. DOI: 10.1515/znc-1973-5-614. View