Detection of Antibodies to U.S. Isolates of Avian Pneumovirus by a Recombinant Nucleocapsid Protein-based Sandwich Enzyme-linked Immunosorbent Assay

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2001 Jul 28

PMID 11474024

Citations 6

Authors

B R Gulati

S Munir

D P Patnayak

S M Goyal

V Kapur

Affiliations

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Abstract

The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the approximately 47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.

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