Protein-RNA Interactions in the Subunits of Human Nuclear RNase P

Overview

Journal RNA

Specialty Molecular Biology

Date 2001 Jul 18

PMID 11455963

Citations 33

Authors

T Jiang

S Altman

Affiliations

Soon will be listed here.

Abstract

A yeast three-hybrid system was employed to analyze interactions in vivo between H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the protein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results of direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.

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Structural organizations of yeast RNase P and RNase MRP holoenzymes as revealed by UV-crosslinking studies of RNA-protein interactions.

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