Isolation of Endonuclease from Mammalian Cells by DNA - Cellulose Chromatography
Overview
Overview
Journal
Nucleic Acids Res
Publisher
Oxford University Press
Specialty
Biochemistry
Date
1975 Jun 1
PMID
1144065
Citations
1
Authors
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
By means of DNA-cellulose chromatography an enzyme with endonucleolytic activity has been isolated from nuclear acidic protein fraction of mammalian cells. The main active fraction, eluted at 0.7 M NaCl, effects the velocity sedimentation of UV-irradiated and alkylated DNA, resulting in a decrease of the molecular weight. The fraction is completely inactive using native as well as heat-denatured DNA.
Citing Articles
Recognition of chemical carcinogen-modified DNA by a DNA-binding protein.
Moranelli F, Lieberman M Proc Natl Acad Sci U S A. 1980; 77(6):3201-5.
PMID: 6932015 PMC: 349582. DOI: 10.1073/pnas.77.6.3201.
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