Two Large Arabidopsis Thaliana Gene Families Are Homologous to the Brassica Gene Superfamily That Encodes Pollen Coat Proteins and the Male Component of the Self-incompatibility Response

Overview

Journal Plant Mol Biol

Date 2001 Jul 5

PMID 11437247

Citations 27

Authors

V Vanoosthuyse

C Miege

C Dumas

J M Cock

Affiliations

Soon will be listed here.

Abstract

The male component of the self-incompatibility response in Brassica has recently been shown to be encoded by the S locus cysteine-rich gene (SCR). SCR is related, at the sequence level, to the pollen coat protein (PCP) gene family whose members encode small, cysteine-rich proteins located in the proteo-lipidic surface layer (tryphine) of Brassica pollen grains. Here we show that the Arabidopsis genome includes two large gene families with homology to SCR and to the PCP gene family, respectively. These genes are poorly predicted by gene-identification algorithms and, with few exceptions, have been missed in previous annotations. Based on sequence comparison and an analysis of the expression patterns of several members of each family, we discuss the possible functions of these genes. In particular, we consider the possibility that SCR-related genes in Arabidopsis may encode ligands for the S gene family of receptor-like kinases in this species.

Citing Articles

Phytosulfokine alpha enhances regeneration of transformed and untransformed protoplasts of .

Vogrincic V, Kastelec D, Murovec J Front Plant Sci. 2024; 15:1379618.

PMID: 38601308 PMC: 11004253. DOI: 10.3389/fpls.2024.1379618.

Comprehensive computational analysis of the SRK-SP11 molecular interaction underlying self-incompatibility in Brassicaceae using improved structure prediction for cysteine-rich proteins.

Sawa T, Moriwaki Y, Jiang H, Murase K, Takayama S, Shimizu K Comput Struct Biotechnol J. 2023; 21:5228-5239.

PMID: 37928947 PMC: 10624595. DOI: 10.1016/j.csbj.2023.10.026.

haplotype collection in Brassicaceae crops-an updated list of haplotypes.

Yamamoto M, Ishii T, Ogura M, Akanuma T, Zhu X, Kitashiba H Breed Sci. 2023; 73(2):132-145.

PMID: 37404351 PMC: 10316313. DOI: 10.1270/jsbbs.22091.

Pollen Coat Proteomes of , , and Reveal Remarkable Diversity of Small Cysteine-Rich Proteins at the Pollen-Stigma Interface.

Wang L, Lau Y, Fan L, Bosch M, Doughty J Biomolecules. 2023; 13(1).

PMID: 36671543 PMC: 9856046. DOI: 10.3390/biom13010157.

Analysis of a gene family for PDF-like peptides from Arabidopsis.

Omidvar R, Vosseler N, Abbas A, Gutmann B, Grunwald-Gruber C, Altmann F Sci Rep. 2021; 11(1):18948.

PMID: 34556705 PMC: 8460643. DOI: 10.1038/s41598-021-98175-6.

References

Pastuglia M, Roby D, Dumas C, Cock J . Rapid induction by wounding and bacterial infection of an S gene family receptor-like kinase gene in Brassica oleracea. Plant Cell. 1997; 9(1):49-60. PMC: 156900. DOI: 10.1105/tpc.9.1.49. View

Usuka J, Brendel V . Gene structure prediction by spliced alignment of genomic DNA with protein sequences: increased accuracy by differential splice site scoring. J Mol Biol. 2000; 297(5):1075-85. DOI: 10.1006/jmbi.2000.3641. View

Murru S, Casula L, Pecorara M, MORI P, Cao A, Pirastu M . Illegitimate recombination produced a duplication within the FVIII gene in a patient with mild hemophilia A. Genomics. 1990; 7(1):115-8. DOI: 10.1016/0888-7543(90)90526-z. View

Kusaba M, Nishio T, Satta Y, Hinata K, Ockendon D . Striking sequence similarity in inter- and intra-specific comparisons of class I SLG alleles from Brassica oleracea and Brassica campestris: implications for the evolution and recognition mechanism. Proc Natl Acad Sci U S A. 1997; 94(14):7673-8. PMC: 23881. DOI: 10.1073/pnas.94.14.7673. View

Doughty J, Dixon S, Hiscock S, Willis A, Parkin I, Dickinson H . PCP-A1, a defensin-like Brassica pollen coat protein that binds the S locus glycoprotein, is the product of gametophytic gene expression. Plant Cell. 1998; 10(8):1333-47. PMC: 144068. DOI: 10.1105/tpc.10.8.1333. View

Cabrillac D, Delorme V, Garin J, Giranton J, Dumas C, Gaude T . The S15 self-incompatibility haplotype in Brassica oleracea includes three S gene family members expressed in stigmas. Plant Cell. 1999; 11(5):971-86. PMC: 144233. DOI: 10.1105/tpc.11.5.971. View

Perriere G, Gouy M . WWW-query: an on-line retrieval system for biological sequence banks. Biochimie. 1996; 78(5):364-9. DOI: 10.1016/0300-9084(96)84768-7. View

Takayama S, Shiba H, Iwano M, Shimosato H, Che F, Kai N . The pollen determinant of self-incompatibility in Brassica campestris. Proc Natl Acad Sci U S A. 2000; 97(4):1920-5. PMC: 26537. DOI: 10.1073/pnas.040556397. View

Luu D, Trick M, Dumas C, Heizmann P . Pollen-stigma adhesion in Brassica spp involves SLG and SLR1 glycoproteins. Plant Cell. 1999; 11(2):251-62. PMC: 144154. DOI: 10.1105/tpc.11.2.251. View

10.

Takayama S, Shiba H, Iwano M, Asano K, Hara M, Che F . Isolation and characterization of pollen coat proteins of Brassica campestris that interact with S locus-related glycoprotein 1 involved in pollen-stigma adhesion. Proc Natl Acad Sci U S A. 2000; 97(7):3765-70. PMC: 16314. DOI: 10.1073/pnas.97.7.3765. View

11.

Fryxell K . The coevolution of gene family trees. Trends Genet. 1996; 12(9):364-9. DOI: 10.1016/s0168-9525(96)80020-5. View

12.

Watanabe M, Ito A, Takada Y, Ninomiya C, Kakizaki T, Takahata Y . Highly divergent sequences of the pollen self-incompatibility (S) gene in class-I S haplotypes of Brassica campestris (syn. rapa) L. FEBS Lett. 2000; 473(2):139-44. DOI: 10.1016/s0014-5793(00)01514-3. View

13.

Dwyer K, Kandasamy M, Mahosky D, Acciai J, Kudish B, Miller J . A superfamily of S locus-related sequences in Arabidopsis: diverse structures and expression patterns. Plant Cell. 1994; 6(12):1829-43. PMC: 160565. DOI: 10.1105/tpc.6.12.1829. View

14.

Luu D, Heizmann P, Dumas C . Pollen-Stigma Adhesion in Kale Is Not Dependent on the Self-(In)Compatibility Genotype. Plant Physiol. 2002; 115(3):1221-1230. PMC: 158587. DOI: 10.1104/pp.115.3.1221. View

15.

Penninckx I, Eggermont K, Terras F, Thomma B, De Samblanx G, Buchala A . Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway. Plant Cell. 1996; 8(12):2309-23. PMC: 161354. DOI: 10.1105/tpc.8.12.2309. View

16.

Walker J . Receptor-like protein kinase genes of Arabidopsis thaliana. Plant J. 1993; 3(3):451-6. DOI: 10.1111/j.1365-313x.1993.tb00164.x. View

17.

Schopfer C, Nasrallah M, Nasrallah J . The male determinant of self-incompatibility in Brassica. Science. 1999; 286(5445):1697-700. DOI: 10.1126/science.286.5445.1697. View

18.

Stein J, Howlett B, Boyes D, Nasrallah M, Nasrallah J . Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci U S A. 1991; 88(19):8816-20. PMC: 52601. DOI: 10.1073/pnas.88.19.8816. View

19.

Boyes D, Nasrallah J . Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea. Mol Gen Genet. 1993; 236(2-3):369-73. DOI: 10.1007/BF00277135. View

20.

Ride J, Davies E, Franklin F, Marshall D . Analysis of Arabidopsis genome sequence reveals a large new gene family in plants. Plant Mol Biol. 1999; 39(5):927-32. DOI: 10.1023/a:1006178511787. View