Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-cell Eggs

Overview

Journal Mol Biotechnol

Publisher Springer

Specialties Biotechnology
Molecular Biology

Date 2001 Jun 9

PMID 11395864

Citations 3

Authors

S Wells

D Murphy

Affiliations

Soon will be listed here.

Abstract

Transgenic technologies that enable rapid movement between genotype and phenotype through specific loss-of-function, overexpression, or misexpression phenotypes will be crucial in the elucidation of gene sequences emerging from genome projects. This article describes detailed procedures for the generation of transgenic mice and rats by the injection of cloned DNA into the pronuclei of fertilized one-cell eggs.

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