Clonable Progenitors Committed to the T Lymphocyte Lineage in the Mouse Bone Marrow; Use of an Extrathymic Pathway

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2001 Jun 8

PMID 11390986

Citations 14

Authors

S Dejbakhsh-Jones

D Zeng

S Strober

Affiliations

Soon will be listed here.

Abstract

We searched for clonable committed T cell progenitors in the adult mouse bone marrow and isolated rare (approximately 0.05%) cells with the Thy-1hiCD2-CD16+CD44hiCD25-Lin- phenotype. In vivo experiments showed that these cells were progenitors committed only to reconstituting the T cell lineage of irradiated Ly5 congenic hosts. Reconstitution of the thymus was minimal compared with that of the bone marrow, spleen, and lymph nodes. At limiting dilutions, donor T cell reconstitution of the spleen frequently occurred without detectable donor cells in the thymus. Progenitors were capable of rapidly reconstituting athymic hosts. In conclusion, the clonable bone marrow progenitors were capable of T cell reconstitution predominantly by means of an extrathymic pathway.

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References

Spangrude G, Heimfeld S, Weissman I . Purification and characterization of mouse hematopoietic stem cells. Science. 1988; 241(4861):58-62. DOI: 10.1126/science.2898810. View

Akashi K, Weissman I . The c-kit+ maturation pathway in mouse thymic T cell development: lineages and selection. Immunity. 1996; 5(2):147-61. DOI: 10.1016/s1074-7613(00)80491-4. View

Zeng D, Lewis D, Dejbakhsh-Jones S, Lan F, Sibley R, Strober S . Bone marrow NK1.1(-) and NK1.1(+) T cells reciprocally regulate acute graft versus host disease. J Exp Med. 1999; 189(7):1073-81. PMC: 2193016. DOI: 10.1084/jem.189.7.1073. View

Rodewald H, Kretzschmar K, Takeda S, Hohl C, Dessing M . Identification of pro-thymocytes in murine fetal blood: T lineage commitment can precede thymus colonization. EMBO J. 1994; 13(18):4229-40. PMC: 395350. DOI: 10.1002/j.1460-2075.1994.tb06743.x. View

Carlyle J, Zuniga-Pflucker J . Requirement for the thymus in alphabeta T lymphocyte lineage commitment. Immunity. 1998; 9(2):187-97. DOI: 10.1016/s1074-7613(00)80601-9. View

Matsuzaki Y, Gyotoku J, Ogawa M, Nishikawa S, Katsura Y, Gachelin G . Characterization of c-kit positive intrathymic stem cells that are restricted to lymphoid differentiation. J Exp Med. 1993; 178(4):1283-92. PMC: 2191219. DOI: 10.1084/jem.178.4.1283. View

Muranski P, Chmielowski B, Ignatowicz L . Mature CD4+ T cells perceive a positively selecting class II MHC/peptide complex in the periphery. J Immunol. 2000; 164(6):3087-94. DOI: 10.4049/jimmunol.164.6.3087. View

Taubenberger J, Reid A, Izon D, Boehme S . Development and characterization of v-myc/v-raf-transformed murine fetal thymocyte cell lines. Cell Immunol. 1996; 171(1):41-7. DOI: 10.1006/cimm.1996.0170. View

Ardavin C, Wu L, Li C, Shortman K . Thymic dendritic cells and T cells develop simultaneously in the thymus from a common precursor population. Nature. 1993; 362(6422):761-3. DOI: 10.1038/362761a0. View

10.

Dejbakhsh-Jones S, Strober S . Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow. Proc Natl Acad Sci U S A. 1999; 96(25):14493-8. PMC: 24464. DOI: 10.1073/pnas.96.25.14493. View

11.

Vremec D, Shortman K . Dendritic cell subtypes in mouse lymphoid organs: cross-correlation of surface markers, changes with incubation, and differences among thymus, spleen, and lymph nodes. J Immunol. 1997; 159(2):565-73. View

12.

Carlyle J, Zuniga-Pflucker J . Lineage commitment and differentiation of T and natural killer lymphocytes in the fetal mouse. Immunol Rev. 1998; 165:63-74. DOI: 10.1111/j.1600-065x.1998.tb01230.x. View

13.

Dejbakhsh-Jones S, Weissman I, Strober S . An alternate pathway for T cell development supported by the bone marrow microenvironment: recapitulation of thymic maturation. J Exp Med. 1998; 187(11):1813-23. PMC: 2212319. DOI: 10.1084/jem.187.11.1813. View

14.

Rodewald H, Awad K, Moingeon P, DAdamio L, Rabinowitz D, Shinkai Y . Fc gamma RII/III and CD2 expression mark distinct subpopulations of immature CD4-CD8- murine thymocytes: in vivo developmental kinetics and T cell receptor beta chain rearrangement status. J Exp Med. 1993; 177(4):1079-92. PMC: 2190966. DOI: 10.1084/jem.177.4.1079. View

15.

Carlyle J, Michie A, Furlonger C, Nakano T, Lenardo M, Paige C . Identification of a novel developmental stage marking lineage commitment of progenitor thymocytes. J Exp Med. 1997; 186(2):173-82. PMC: 2198984. DOI: 10.1084/jem.186.2.173. View

16.

Schmidt-Wolf I, Liang O, Dejbakhsh-Jones S, Wang H, Cheng L, Holm B . Homogeneous antigen receptor beta-chain genes in cloned CD4- CD8- alpha beta T suppressor cells. J Immunol. 1993; 151(10):5348-53. View

17.

Uchida N, Weissman I . Searching for hematopoietic stem cells: evidence that Thy-1.1lo Lin- Sca-1+ cells are the only stem cells in C57BL/Ka-Thy-1.1 bone marrow. J Exp Med. 1992; 175(1):175-84. PMC: 2119064. DOI: 10.1084/jem.175.1.175. View

18.

Kondo M, Weissman I, Akashi K . Identification of clonogenic common lymphoid progenitors in mouse bone marrow. Cell. 1997; 91(5):661-72. DOI: 10.1016/s0092-8674(00)80453-5. View

19.

Beutner U, MacDonald H . TCR-MHC class II interaction is required for peripheral expansion of CD4 cells in a T cell-deficient host. Int Immunol. 1998; 10(3):305-10. DOI: 10.1093/intimm/10.3.305. View

20.

Saint-Ruf C, Ungewiss K, Groettrup M, Bruno L, Fehling H, von Boehmer H . Analysis and expression of a cloned pre-T cell receptor gene. Science. 1994. 266: 1208-1212. J Immunol. 2009; 182(9):5165-9. View