Overexpression of P27Kip1 Lengthens the G1 Phase in a Mouse Model That Targets Inducible Gene Expression to Central Nervous System Progenitor Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2001 May 24

PMID 11371649

Citations 43

Authors

T Mitsuhashi

Y Aoki

Y Z Eksioglu

T Takahashi

P G Bhide

S A Reeves

V S Caviness Jr

Affiliations

Soon will be listed here.

Abstract

We describe a mouse model in which p27(Kip1) transgene expression is spatially restricted to the central nervous system neuroepithelium and temporally controlled with doxycycline. Transgene-specific transcripts are detectable within 6 h of doxycycline administration, and maximum nonlethal expression is approached within 12 h. After 18-26 h of transgene expression, the G(1) phase of the cell cycle is estimated to increase from 9 to 13 h in the neocortical neuroepithelium, the maximum G(1) phase length attainable in this proliferative population in normal mice. Thus our data establish a direct link between p27(Kip1) and control of G(1) phase length in the mammalian central nervous system and unveil intrinsic mechanisms that constrain the G(1) phase length to a putative physiological maximum despite ongoing p27(Kip1) transgene expression.

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