Quantitative Monitoring of Circulating Epstein-Barr Virus DNA for Predicting the Development of Posttransplantation Lymphoproliferative Disease

Overview

Journal Int J Hematol

Specialty Hematology

Date 2001 May 10

PMID 11345197

Citations 8

Authors

S Ohga

E Kubo

A Nomura

H Takada

N Suga

E Ishii

A Suminoe

T Inamitsu

A Matsuzaki

N Kasuga

T Hara

Affiliations

Soon will be listed here.

Abstract

Epstein-Barr virus (EBV)-DNA was quantitatively measured to assess posttransplantation virus reactivation by real-time polymerase chain reaction (PCR). In the first retrospective analysis of a 7-year-old boy with lymphoproliferative disease (LPD) after an unrelated cord blood transplantation, serum EBV-DNA progressively increased to 4 x 10(5) copies/mL. EBV load was then prospectively monitored in peripheral blood from posttransplantation patients. The second case was an 8 year-old boy with aplastic anemia who received a CD34+ cell transplantation. This patient died of LPD with the progression of pulmonary nodules. EBV-DNA increased to 4 x 10(4) copies/mL after the control of cytomegalovirus reactivation. On the other hand, EBV-DNA was undetectable (<200 copies/mL) in the series of all 58 samples from 10 patients who did not develop LPD after hematopoietic stem cell transplantation. Sequential monitoring of circulating EBV-DNA by quantitative PCR may be a useful indicator for predicting the development of posttransplantation LPD.

Citing Articles

Secondary B-cell lymphoma associated with the Epstein-Barr virus in chronic lymphocytic leukemia patients.

Morscio J, Bittoun E, Volders N, Lurquin E, Wlodarska I, Gheysens O J Hematop. 2018; 9:113-120.

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Late-onset fatal Epstein-Barr virus-associated hemophagocytic syndrome following cord blood cell transplantation for adult acute lymphoblastic leukemia.

Kawabata Y, Hirokawa M, Saitoh Y, Kosugi S, Yoshioka T, Fujishima M Int J Hematol. 2006; 84(5):445-8.

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Marked increased risk of Epstein-Barr virus-related complications with the addition of antithymocyte globulin to a nonmyeloablative conditioning prior to unrelated umbilical cord blood transplantation.

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High fatality rate of Epstein-Barr virus-associated lymphoproliferative disorder occurring after bone marrow transplantation with rabbit antithymocyte globulin conditioning regimens.

Peres E, Savasan S, Klein J, Abidi M, Dansey R, Abella E J Clin Microbiol. 2005; 43(7):3540-3.

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Surface immunoglobulin-deficient Epstein-Barr virus-infected B cells in the peripheral blood of pediatric solid-organ transplant recipients.

Schauer E, Webber S, Green M, Rowe D J Clin Microbiol. 2004; 42(12):5802-10.

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References

Kimura H, Morita M, Yabuta Y, Kuzushima K, Kato K, Kojima S . Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. J Clin Microbiol. 1998; 37(1):132-6. PMC: 84187. DOI: 10.1128/JCM.37.1.132-136.1999. View

Lucas K, Pollok K, Emanuel D . Post-transplant EBV induced lymphoproliferative disorders. Leuk Lymphoma. 1997; 25(1-2):1-8. DOI: 10.3109/10428199709042491. View

Manez R, Breinig M, Linden P, Wilson J, Torre-Cisneros J, Kusne S . Posttransplant lymphoproliferative disease in primary Epstein-Barr virus infection after liver transplantation: the role of cytomegalovirus disease. J Infect Dis. 1997; 176(6):1462-7. DOI: 10.1086/514142. View

Gustafsson A, Levitsky V, Zou J, Frisan T, Dalianis T, Ljungman P . Epstein-Barr virus (EBV) load in bone marrow transplant recipients at risk to develop posttransplant lymphoproliferative disease: prophylactic infusion of EBV-specific cytotoxic T cells. Blood. 2000; 95(3):807-14. View

Sun Q, Pollok K, Burton R, Dai L, Britt W, Emanuel D . Simultaneous ex vivo expansion of cytomegalovirus and Epstein-Barr virus-specific cytotoxic T lymphocytes using B-lymphoblastoid cell lines expressing cytomegalovirus pp65. Blood. 1999; 94(9):3242-50. View

Ohga S, Nomura A, Takada H, Ihara K, Kawakami K, Yanai F . Epstein-Barr virus (EBV) load and cytokine gene expression in activated T cells of chronic active EBV infection. J Infect Dis. 2000; 183(1):1-7. DOI: 10.1086/317653. View

Sanburn N, Cornetta K . Rapid titer determination using quantitative real-time PCR. Gene Ther. 1999; 6(7):1340-5. DOI: 10.1038/sj.gt.3300948. View

Maeda A, Wakiguchi H, Yokoyama W, Hisakawa H, Tomoda T, Kurashige T . Persistently high Epstein-Barr virus (EBV) loads in peripheral blood lymphocytes from patients with chronic active EBV infection. J Infect Dis. 1999; 179(4):1012-5. DOI: 10.1086/314691. View

Ohga S, Kimura N, Takada H, Nagano M, Ohshima K, Nomura A . Restricted diversification of T-cells in chronic active Epstein-Barr virus infection: potential inclination to T-lymphoproliferative disease. Am J Hematol. 1999; 61(1):26-33. DOI: 10.1002/(sici)1096-8652(199905)61:1<26::aid-ajh6>3.0.co;2-r. View

10.

Machida U, Kami M, Fukui T, Kazuyama Y, Kinoshita M, Tanaka Y . Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation. J Clin Microbiol. 2000; 38(7):2536-42. PMC: 86962. DOI: 10.1128/JCM.38.7.2536-2542.2000. View

11.

Lucas K, Burton R, Zimmerman S, Wang J, Cornetta K, Robertson K . Semiquantitative Epstein-Barr virus (EBV) polymerase chain reaction for the determination of patients at risk for EBV-induced lymphoproliferative disease after stem cell transplantation. Blood. 1998; 91(10):3654-61. View

12.

Ohga S, Kanaya Y, Maki H, Takada H, Ohshima K, Kanda M . Epstein-Barr virus-associated lymphoproliferative disease after a cord blood transplant for Diamond-Blackfan anemia. Bone Marrow Transplant. 2000; 25(2):209-12. DOI: 10.1038/sj.bmt.1702138. View

13.

Nalesnik M . Clinical and pathological features of post-transplant lymphoproliferative disorders (PTLD). Springer Semin Immunopathol. 1998; 20(3-4):325-42. DOI: 10.1007/BF00838047. View

14.

Walker R, Marshall W, Strickler J, Wiesner R, Velosa J, Habermann T . Pretransplantation assessment of the risk of lymphoproliferative disorder. Clin Infect Dis. 1995; 20(5):1346-53. DOI: 10.1093/clinids/20.5.1346. View

15.

Knowles D . Immunodeficiency-associated lymphoproliferative disorders. Mod Pathol. 1999; 12(2):200-17. View