Transport Systems for Branched-chain Amino Acids in Pseudomonas Aeruginosa

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1979 Sep 1

PMID 113383

Citations 30

Authors

T Hoshino

Affiliations

Soon will be listed here.

Abstract

The cells of Pseudomonas aeruginosa showed high activity for leucine transport in the absence of Na+, giving a Km value of 0.34 microM. In the presence of Na+, however, two Km values, 0.37 microM (LIV-I system) and 7.6 microM (LIV-II system), were obtained. The former system seemed to serve not only for the entry of leucine, isoleucine, and valine, but also for that of alanine and threonine, although less effectively. However, the LIV-II system served for the entry of branched-chain amino acids only. The LIV-II system alone was operative in membrane vesicles, for the transport of branched-chain amino acids in membrane vesicles required Na+ and gave single Km values for the respective amino acids. When cells were osmotically shocked, the activity of the LIV-I system decreased, whereas the LIV-II system remained unaffected. The shock fluid from P. aeruginosa cells showed leucine-binding activity with a dissociation constant of 0.25 microM. The specificity of the activity was very similar to that of the LIV-I system. These results suggest that a leucine-binding protein(s) in the periplasmic space may be required for the transport process via the LIV-I system of P. aeruginosa.

Citing Articles

Evolution of two metabolic genes involved in nucleotide and amino acid metabolism in Pseudomonas aeruginosa.

Wu Y, Shi Y, Liang X PLoS One. 2024; 19(12):e0315931.

PMID: 39689084 PMC: 11651626. DOI: 10.1371/journal.pone.0315931.

Improving L-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX.

Wang S, Fang Y, Wang Z, Zhang S, Wang L, Guo Y Microb Cell Fact. 2021; 20(1):58.

PMID: 33653345 PMC: 7927397. DOI: 10.1186/s12934-021-01546-x.

Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta.

Moeller F, Webster N, Herbold C, Behnam F, Domman D, Albertsen M Environ Microbiol. 2019; 21(10):3831-3854.

PMID: 31271506 PMC: 6790972. DOI: 10.1111/1462-2920.14732.

Genome-scale metabolic modeling of responses to polymyxins in Pseudomonas aeruginosa.

Zhu Y, Czauderna T, Zhao J, Klapperstueck M, Mahamad Maifiah M, Han M Gigascience. 2018; 7(4).

PMID: 29688451 PMC: 6333913. DOI: 10.1093/gigascience/giy021.

Tension-activated channels in the mechanism of osmotic fitness in .

Cetiner U, Rowe I, Schams A, Mayhew C, Rubin D, Anishkin A J Gen Physiol. 2017; 149(5):595-609.

PMID: 28424229 PMC: 5412531. DOI: 10.1085/jgp.201611699.

References

Wood J . Leucine transport in Escherichia coli. The resolution of multiple transport systems and their coupling to metabolic energy. J Biol Chem. 1975; 250(12):4477-85. View

Fein J, MacLEOD R . Characterization of neutral amino acid transport in a marine pseudomonad. J Bacteriol. 1975; 124(3):1177-90. PMC: 236025. DOI: 10.1128/jb.124.3.1177-1190.1975. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Bhatti A, DeVoe I, Ingram J . The release and characterization of some periplasm-located enzymes of Pseudomona aeruginosa. Can J Microbiol. 1976; 22(10):1425-9. DOI: 10.1139/m76-211. View

Kiritani K, Ohnishi K . Repression and inhibition of transport systems for branched-chain amino acids in Salmonella typhimurium. J Bacteriol. 1977; 129(2):589-98. PMC: 234983. DOI: 10.1128/jb.129.2.589-598.1977. View

Yamato I, Anraku Y . Transport of sugars and amino acids in bacteria. XVIII. Properties of an isoleucine carrier in the cytoplasmic membrane vesicles of Escherichia coli. J Biochem. 1977; 81(5):1517-23. View

Stinson M, Cohen M, MERRICK J . Purification and properties of the periplasmic glucose-binding protein of Pseudomonas aeruginosa. J Bacteriol. 1977; 131(2):672-81. PMC: 235478. DOI: 10.1128/jb.131.2.672-681.1977. View

Cheng K, Ingram J, Costerton J . Interactions of alkaline phosphatase and the cell wall of Pseudomonas aeruginosa. J Bacteriol. 1971; 107(1):325-36. PMC: 246919. DOI: 10.1128/jb.107.1.325-336.1971. View

Kobayashi H, KIN E, Anraku Y . Transport of sugars and amino acids in bacteria. X. Sources of energy and energy coupling reactions of the active transport systems for isoleucine and proline in E. coli. J Biochem. 1974; 76(2):251-61. DOI: 10.1093/oxfordjournals.jbchem.a130567. View

10.

OXENDER D . Membrane transport. Annu Rev Biochem. 1972; 41(10):777-814. DOI: 10.1146/annurev.bi.41.070172.004021. View

11.

Rahmanian M, Claus D, OXENDER D . Multiplicity of leucine transport systems in Escherichia coli K-12. J Bacteriol. 1973; 116(3):1258-66. PMC: 246482. DOI: 10.1128/jb.116.3.1258-1266.1973. View

12.

Guardiola J, De Felice M, Klopotowski T, Iaccarino M . Multiplicity of isoleucine, leucine, and valine transport systems in Escherichia coli K-12. J Bacteriol. 1974; 117(2):382-92. PMC: 285525. DOI: 10.1128/jb.117.2.382-392.1974. View

13.

KAY W, Gronlund A . Influence of carbon or nitrogen starvation on amino acid transport in Pseudomonas aeruginosa. J Bacteriol. 1969; 100(1):276-82. PMC: 315389. DOI: 10.1128/jb.100.1.276-282.1969. View

14.

Tsay S, Brown K, Gaudy E . Transport of glycerol by Pseudomonas aeruginosa. J Bacteriol. 1971; 108(1):82-8. PMC: 247035. DOI: 10.1128/jb.108.1.82-88.1971. View

15.

Neal J . Analysis of Michaelis kinetics for two independent, saturable membrane transport functions. J Theor Biol. 1972; 35(1):113-8. DOI: 10.1016/0022-5193(72)90196-8. View

16.

Stinson M, Cohen M, MERRICK J . Isolation of dicarboxylic acid- and glucose-binding proteins from Pseudomonas aeruginosa. J Bacteriol. 1976; 128(2):573-9. PMC: 232792. DOI: 10.1128/jb.128.2.573-579.1976. View

17.

Hoshino T, Kageyama M . Sodium-dependent transport of L-leucine in membrane vesicles prepared from Pseudomonas aeruginosa. J Bacteriol. 1979; 137(1):73-81. PMC: 218420. DOI: 10.1128/jb.137.1.73-81.1979. View

18.

Pearce S, Hildebrandt V, Lee T . Third system for neutral amino acid transport in a marine pseudomonad. J Bacteriol. 1977; 130(1):37-47. PMC: 235171. DOI: 10.1128/jb.130.1.37-47.1977. View