Optimization of Second-harmonic Generation Microscopy

Overview

Journal Micron

Date 2001 May 4

PMID 11334739

Citations 20

Authors

R Gauderon

P B Lukins

C J Sheppard

Affiliations

Soon will be listed here.

Abstract

We describe the principles and characteristics of second-harmonic generation imaging (SHGI) and explore various methods for optimization of the technique. Second-harmonic imaging is optimized for ultrashort laser pulses, high numerical aperture microscope objectives, a highly sensitive non-descanned large area detector, pseudo-phase-matching, and specimens with large second-order non linearity or which exhibit surface plasmon enhanced phenomena. We also compare and contrast the techniques of SHGI and two-photon excited fluorescence imaging.

Citing Articles

Quantifying collagen reorganization during immunotherapy in murine melanoma with second harmonic generation imaging.

Heaton A, Burkard N, Sondel P, Skala M Biophotonics Discov. 2024; 1(1).

PMID: 39011049 PMC: 11247620. DOI: 10.1117/1.bios.1.1.015004.

On the quantitative analysis of lamellar collagen arrangement with second-harmonic generation imaging.

Guimaraes P, Morgado M, Batista A Biomed Opt Express. 2024; 15(4):2666-2680.

PMID: 38633085 PMC: 11019681. DOI: 10.1364/BOE.516817.

Two-Photon Imaging for Non-Invasive Corneal Examination.

Batista A, Guimaraes P, Domingues J, Quadrado M, Morgado A Sensors (Basel). 2022; 22(24).

PMID: 36560071 PMC: 9783858. DOI: 10.3390/s22249699.

Alterations of bone material properties in growing Ifitm5/BRIL p.S42 knock-in mice, a new model for atypical type VI osteogenesis imperfecta.

Hedjazi G, Guterman-Ram G, Blouin S, Schemenz V, Wagermaier W, Fratzl P Bone. 2022; 162:116451.

PMID: 35654352 PMC: 11162744. DOI: 10.1016/j.bone.2022.116451.

Nonlinear Optical Methods for Characterization of Molecular Structure and Surface Chemistry.

Johansson P, Schmuser L, Castner D Top Catal. 2018; 61(9-11):1101-1124.

PMID: 29955207 PMC: 6018028. DOI: 10.1007/s11244-018-0924-3.