Structure and Function of the C-terminal PABC Domain of Human Poly(A)-binding Protein

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2001 Apr 5

PMID 11287632

Citations 95

Authors

G Kozlov

J F Trempe

K Khaleghpour

A Kahvejian

I Ekiel

K Gehring

Affiliations

Soon will be listed here.

Abstract

We have determined the solution structure of the C-terminal quarter of human poly(A)-binding protein (hPABP). The protein fragment contains a protein domain, PABC [for poly(A)-binding protein C-terminal domain], which is also found associated with the HECT family of ubiquitin ligases. By using peptides derived from PABP interacting protein (Paip) 1, Paip2, and eRF3, we show that PABC functions as a peptide binding domain. We use chemical shift perturbation analysis to identify the peptide binding site in PABC and the major elements involved in peptide recognition. From comparative sequence analysis of PABC-binding peptides, we formulate a preliminary PABC consensus sequence and identify human ataxin-2, the protein responsible for type 2 spinocerebellar ataxia (SCA2), as a potential PABC ligand.

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