Histochemical Localisation of Versican, Aggrecan and Hyaluronan in the Developing Condylar Cartilage of the Fetal Rat Mandible

Overview

Journal J Anat

Specialty Anatomy & Histology

Date 2001 Mar 29

PMID 11273038

Citations 9

Authors

S Shibata

K Fukada

S Suzuki

T Ogawa

Y Yamashita

Affiliations

Soon will be listed here.

Abstract

We investigated the histochemical localisation of versican, aggrecan and hyaluronan in the developing condylar cartilage of the fetal rat mandible at d 15-17 of gestation. At d 15 of gestation, immunostaining for versican was detected in the anlage of the future condylar process (condylar anlage), although the staining intensity showed a considerable regional variation. At d 16 of gestation, a metachromatically stained matrix firstly appeared in the condylar anlage. Aggrecan, hyaluronan and versican were simultaneously detected in this newly formed condylar cartilage. At d 17 of gestation, immunostaining for versican became restricted to the perichondrium and was barely detected in the cartilage. Colocalisation of versican and aggrecan was also seen in the cranial base cartilage at d 14 of gestation. These results indicate that although versican is replaced by aggrecan during the transition from prechondrogenic tissue to cartilage, both molecules were temporally colocalised in the newly formed cartilage. A hyaluronan-rich, low-versican area was identified in the posterior end of the condylar anlage during d 15-17 of gestation. The existence of this area is a unique structural feature of the developing condylar cartilage.

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References

Ishii M, Suda N, Tengan T, Suzuki S, Kuroda T . Immunohistochemical findings type I and type II collagen in prenatal mouse mandibular condylar cartilage compared with the tibial anlage. Arch Oral Biol. 1998; 43(7):545-50. DOI: 10.1016/s0003-9969(98)00028-4. View

Shibata S, Fukada K, Suzuki S, Yamashita Y . Immunohistochemistry of collagen types II and X, and enzyme-histochemistry of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible. J Anat. 1998; 191 ( Pt 4):561-70. PMC: 1467723. DOI: 10.1046/j.1469-7580.1997.19140561.x. View

Fukada K, Shibata S, Suzuki S, Ohya K, Kuroda T . In situ hybridisation study of type I, II, X collagens and aggrecan mRNas in the developing condylar cartilage of fetal mouse mandible. J Anat. 1999; 195 ( Pt 3):321-9. PMC: 1468002. DOI: 10.1046/j.1469-7580.1999.19530321.x. View

Shibata S, Yoneda S, Yanagishita M, Yamashita Y . Isolation of proteoglycan (versican) aggregate from rat dental pulp. Arch Oral Biol. 2000; 45(7):563-8. DOI: 10.1016/s0003-9969(00)00023-6. View

Kimata K, Oike Y, Tani K, Shinomura T, Yamagata M, Uritani M . A large chondroitin sulfate proteoglycan (PG-M) synthesized before chondrogenesis in the limb bud of chick embryo. J Biol Chem. 1986; 261(29):13517-25. View

Silbermann M, Reddi A, Hand A, Leapman R, von der Mark K, Franzen A . Further characterisation of the extracellular matrix in the mandibular condyle in neonatal mice. J Anat. 1987; 151:169-88. PMC: 1261710. View

Luder H, Leblond C, von der Mark K . Cellular stages in cartilage formation as revealed by morphometry, radioautography and type II collagen immunostaining of the mandibular condyle from weanling rats. Am J Anat. 1988; 182(3):197-214. DOI: 10.1002/aja.1001820302. View

Zimmermann D, Ruoslahti E . Multiple domains of the large fibroblast proteoglycan, versican. EMBO J. 1989; 8(10):2975-81. PMC: 401368. DOI: 10.1002/j.1460-2075.1989.tb08447.x. View

Shinomura T, Jensen K, Yamagata M, Kimata K, Solursh M . The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth. Anat Embryol (Berl). 1990; 181(3):227-33. DOI: 10.1007/BF00174617. View

10.

Asher R, Perides G, Vanderhaeghen J, Bignami A . Extracellular matrix of central nervous system white matter: demonstration of an hyaluronate-protein complex. J Neurosci Res. 1991; 28(3):410-21. DOI: 10.1002/jnr.490280314. View

11.

LeBaron R, Zimmermann D, Ruoslahti E . Hyaluronate binding properties of versican. J Biol Chem. 1992; 267(14):10003-10. View

12.

Calabro A, Hascall V, Caterson B . Monoclonal antibodies directed against epitopes within the core protein structure of the large aggregating proteoglycan (aggrecan) from the swarm rat chondrosarcoma. Arch Biochem Biophys. 1992; 298(2):349-60. DOI: 10.1016/0003-9861(92)90421-r. View

13.

Asari A, Miyauchi S, Miyazaki K, Hamai A, Horie K, Takahashi T . Intra- and extracellular localization of hyaluronic acid and proteoglycan constituents (chondroitin sulfate, keratan sulfate, and protein core) in articular cartilage of rabbit tibia. J Histochem Cytochem. 1992; 40(11):1693-704. DOI: 10.1177/40.11.1431058. View

14.

Bignami A, Perides G, Rahemtulla F . Versican, a hyaluronate-binding proteoglycan of embryonal precartilaginous mesenchyma, is mainly expressed postnatally in rat brain. J Neurosci Res. 1993; 34(1):97-106. DOI: 10.1002/jnr.490340110. View

15.

Yamagata M, Shinomura T, Kimata K . Tissue variation of two large chondroitin sulfate proteoglycans (PG-M/versican and PG-H/aggrecan) in chick embryos. Anat Embryol (Berl). 1993; 187(5):433-44. DOI: 10.1007/BF00174419. View

16.

Landolt R, Vaughan L, Winterhalter K, Zimmermann D . Versican is selectively expressed in embryonic tissues that act as barriers to neural crest cell migration and axon outgrowth. Development. 1995; 121(8):2303-12. DOI: 10.1242/dev.121.8.2303. View

17.

Bode-Lesniewska B, Dours-Zimmermann M, Odermatt B, Briner J, Heitz P, Zimmermann D . Distribution of the large aggregating proteoglycan versican in adult human tissues. J Histochem Cytochem. 1996; 44(4):303-12. DOI: 10.1177/44.4.8601689. View

18.

Miyake T, Cameron A, Hall B . Stage-specific expression patterns of alkaline phosphatase during development of the first arch skeleton in inbred C57BL/6 mouse embryos. J Anat. 1997; 190 ( Pt 2):239-60. PMC: 1467603. DOI: 10.1046/j.1469-7580.1997.19020239.x. View

19.

Yamauchi S, Cheng H, NEAME P, Caterson B, Yamauchi M . Identification, partial characterization, and distribution of versican and link protein in bovine dental pulp. J Dent Res. 1997; 76(11):1730-6. DOI: 10.1177/00220345970760110301. View

20.

Shibata S, Kaneko S, Yanagishita M, Yamashita Y . Histochemical localization of hyaluronan and versican in the rat molar dental pulp. Arch Oral Biol. 1999; 44(4):373-6. DOI: 10.1016/s0003-9969(98)00110-1. View