Histochemical Differentiation of Autometallographically Traceable Metals (Au, Ag, Hg, Bi, Zn): Protocols for Chemical Removal of Separate Autometallographic Metal Clusters in Epon Sections

Overview

Journal Histochem J

Specialty Biochemistry

Date 2001 Mar 29

PMID 11272803

Citations 11

Authors

M Stoltenberg

G Danscher

Affiliations

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Abstract

Nano-sized clusters of gold atoms, or alternatively silver, mercury, bismuth, or zinc sulphide/selenide molecules, can be autometallographically silver-enhanced by being placed in a developer containing reducing molecules and silver ions, i.e. an autometallographic developer. A specific recipe has been worked out for each autometallographically traceable metal, and in cases where two or more autometallographic catalysts are present in the same section it is feasible to distinguish one from the other by chemical removal of one or the other of the metals. In the present study we present protocols that allow differentiation and control of specificity of the established autometallographically detectable metals. It is recommended to implement a multi-element analysis, e.g. proton-induced X-ray emission on a few samples to secure the histochemical data.

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References

Stoltenberg M, Danscher G, Pamphlett R, Christensen M, Rungby J . Histochemical tracing of bismuth in testis from rats exposed intraperitoneally to bismuth subnitrate. Reprod Toxicol. 2000; 14(1):65-71. DOI: 10.1016/s0890-6238(99)00060-x. View

Yuan X . Apoptotic macrophage-derived foam cells of human atheromas are rich in iron and ferritin, suggesting iron-catalysed reactions to be involved in apoptosis. Free Radic Res. 2000; 30(3):221-31. DOI: 10.1080/10715769900300241. View

Domouhtsidou G, Dimitriadis V . Ultrastructural localization of heavy metals (Hg, Ag, Pb, and Cu) in gills and digestive gland of mussels, Mytilus galloprovincialis (L.). Arch Environ Contam Toxicol. 2000; 38(4):472-8. DOI: 10.1007/s002449910062. View

Pamphlett R, Stoltenberg M, Rungby J, Danscher G . Uptake of bismuth in motor neurons of mice after single oral doses of bismuth compounds. Neurotoxicol Teratol. 2000; 22(4):559-63. DOI: 10.1016/s0892-0362(00)00083-0. View

Danscher G, Stoltenberg M, Kemp K, Pamphlett R . Bismuth autometallography: protocol, specificity, and differentiation. J Histochem Cytochem. 2000; 48(11):1503-10. DOI: 10.1177/002215540004801107. View

Christensen M, Frederickson C, Danscher G . Retrograde tracing of zinc-containing neurons by selenide ions: a survey of seven selenium compounds. J Histochem Cytochem. 1992; 40(4):575-9. DOI: 10.1177/40.4.1313065. View

TIMM F . [Histochemistry of heavy metals; the sulfide-silver procedure]. Dtsch Z Gesamte Gerichtl Med. 1958; 46(5):706-11. View

Danscher G . Histochemical tracing of zinc, mercury, silver and gold. Prog Histochem Cytochem. 1991; 23(1-4):273-85. DOI: 10.1016/s0079-6336(11)80196-8. View

Moller-Madsen B, Danscher G . Localization of mercury in CNS of the rat. IV. The effect of selenium on orally administered organic and inorganic mercury. Toxicol Appl Pharmacol. 1991; 108(3):457-73. DOI: 10.1016/0041-008x(91)90092-s. View

10.

Howell G, Frederickson C, Danscher G . Evidence from dithizone and selenium zinc histochemistry that perivascular mossy fiber boutons stain preferentially "in vivo". Histochemistry. 1989; 92(2):121-5. DOI: 10.1007/BF00490230. View

11.

Danscher G, Moller-Madsen B . Silver amplification of mercury sulfide and selenide: a histochemical method for light and electron microscopic localization of mercury in tissue. J Histochem Cytochem. 1985; 33(3):219-28. DOI: 10.1177/33.3.2579122. View

12.

Lormee P, Lecolle S, Septier D, Le Denmat D, Goldberg M . Autometallography for histochemical visualization of rat incisor polyanions with cuprolinic blue. J Histochem Cytochem. 1989; 37(2):203-8. DOI: 10.1177/37.2.2642940. View

13.

Christensen M, Rungby J, Mogensen S . Effects of selenium on toxicity and ultrastructural localization of mercury in cultured murine macrophages. Toxicol Lett. 1989; 47(3):259-70. DOI: 10.1016/0378-4274(89)90144-6. View

14.

Danscher G . Can aluminium be visualized in CNS by silver amplification?. Acta Neuropathol. 1988; 76(1):107. DOI: 10.1007/BF00687687. View

15.

Rungby J, Ellermann-Eriksen S, Danscher G . Effects of selenium on toxicity and ultrastructural localization of silver in cultured macrophages. Arch Toxicol. 1987; 61(1):40-5. DOI: 10.1007/BF00324546. View

16.

Rungby J . Silver-induced lipid peroxidation in mice: interactions with selenium and nickel. Toxicology. 1987; 45(2):135-42. DOI: 10.1016/0300-483x(87)90099-0. View

17.

Danscher G, Rungby J . Differentiation of histochemically visualized mercury and silver. Histochem J. 1986; 18(2-3):109-14. DOI: 10.1007/BF01675364. View

18.

BRUNK U, Brun A . Histochemical evidence for lysosomal uptake of lead in tissue cultured fibroblasts. Histochemie. 1972; 29(2):140-6. DOI: 10.1007/BF00277282. View

19.

Danscher G, Haug F, Fredens K . Effect of diethyldithiocarbamate (DEDTC) on sulphide silver stained boutons. Reversible blocking of Timm's sulphide silver stain for "heavy" metals in DEDTC treated rats (light microscopy). Exp Brain Res. 1973; 16(5):521-32. DOI: 10.1007/BF00234478. View

20.

Iwata H, Okamoto H, Ohsawa Y . Effect of selenium on methylmercury poisoning. Res Commun Chem Pathol Pharmacol. 1973; 5(3):673-80. View