Coordinated Response of Mammalian Rad51 and Rad52 to DNA Damage

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2001 Mar 21

PMID 11256631

Citations 36

Authors

Y Liu

N Maizels

Affiliations

Soon will be listed here.

Abstract

Biochemical analysis has shown that mammalian Rad51 and Rad52 interact and synergize in DNA recombination reactions in vitro, but these proteins have not been shown to function together in response to DNA damage in vivo. By analysis of murine cells expressing murine Rad52 tagged with green fluorescent protein (GFP)-Rad52, we now show that DNA damage causes Rad51 and GFP-Rad52 to colocalize in distinct nuclear foci. Cells expressing GFP-Rad52 show both increased survival and an increased number of Rad51 foci, raising the possibility that Rad52 is limiting for repair. These observations provide evidence of coordinated function of Rad51 and Rad52 in vivo and support the hypothesis that Rad52 plays an important role in the DNA damage response in mammalian cells.

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References

Liu Y, Li M, Lee E, Maizels N . Localization and dynamic relocalization of mammalian Rad52 during the cell cycle and in response to DNA damage. Curr Biol. 1999; 9(17):975-8. DOI: 10.1016/s0960-9822(99)80427-8. View

Nelms B, Maser R, Mackay J, Lagally M, Petrini J . In situ visualization of DNA double-strand break repair in human fibroblasts. Science. 1998; 280(5363):590-2. DOI: 10.1126/science.280.5363.590. View

Liang F, Han M, Romanienko P, Jasin M . Homology-directed repair is a major double-strand break repair pathway in mammalian cells. Proc Natl Acad Sci U S A. 1998; 95(9):5172-7. PMC: 20233. DOI: 10.1073/pnas.95.9.5172. View

Sugiyama T, New J, Kowalczykowski S . DNA annealing by RAD52 protein is stimulated by specific interaction with the complex of replication protein A and single-stranded DNA. Proc Natl Acad Sci U S A. 1998; 95(11):6049-54. PMC: 27583. DOI: 10.1073/pnas.95.11.6049. View

Brizard F, Le Coniat M, Radford I, Berger R, Brizard A . Abnormalities of the short arm of chromosome 12 in T cell prolymphocytic leukemia. Leukemia. 1998; 12(6):972-5. DOI: 10.1038/sj.leu.2401034. View

Adzuma K, Ogawa T, Ogawa H . Primary structure of the RAD52 gene in Saccharomyces cerevisiae. Mol Cell Biol. 1984; 4(12):2735-44. PMC: 369283. DOI: 10.1128/mcb.4.12.2735-2744.1984. View

Gottlieb S, Esposito R . A new role for a yeast transcriptional silencer gene, SIR2, in regulation of recombination in ribosomal DNA. Cell. 1989; 56(5):771-6. DOI: 10.1016/0092-8674(89)90681-8. View

Lin Y, Keil R . Mutations affecting RNA polymerase I-stimulated exchange and rDNA recombination in yeast. Genetics. 1991; 127(1):31-8. PMC: 1204309. DOI: 10.1093/genetics/127.1.31. View

Shinohara A, Ogawa H, Ogawa T . Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell. 1992; 69(3):457-70. DOI: 10.1016/0092-8674(92)90447-k. View

10.

Milne G, Weaver D . Dominant negative alleles of RAD52 reveal a DNA repair/recombination complex including Rad51 and Rad52. Genes Dev. 1993; 7(9):1755-65. DOI: 10.1101/gad.7.9.1755. View

11.

Donovan J, Milne G, Weaver D . Homotypic and heterotypic protein associations control Rad51 function in double-strand break repair. Genes Dev. 1994; 8(21):2552-62. DOI: 10.1101/gad.8.21.2552. View

12.

Muris D, Bezzubova O, Buerstedde J, Vreeken K, Balajee A, Osgood C . Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination. Mutat Res. 1994; 315(3):295-305. DOI: 10.1016/0921-8777(94)90040-x. View

13.

Kowalczykowski S, Eggleston A . Homologous pairing and DNA strand-exchange proteins. Annu Rev Biochem. 1994; 63:991-1043. DOI: 10.1146/annurev.bi.63.070194.005015. View

14.

Haaf T, Golub E, Reddy G, Radding C, Ward D . Nuclear foci of mammalian Rad51 recombination protein in somatic cells after DNA damage and its localization in synaptonemal complexes. Proc Natl Acad Sci U S A. 1995; 92(6):2298-302. PMC: 42471. DOI: 10.1073/pnas.92.6.2298. View

15.

Shen Z, Denison K, LOBB R, Gatewood J, Chen D . The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues. Genomics. 1995; 25(1):199-206. DOI: 10.1016/0888-7543(95)80126-7. View

16.

Park M . Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells. J Biol Chem. 1995; 270(26):15467-70. DOI: 10.1074/jbc.270.26.15467. View

17.

Ashley T, Plug A, Xu J, Solari A, Reddy G, Golub E . Dynamic changes in Rad51 distribution on chromatin during meiosis in male and female vertebrates. Chromosoma. 1995; 104(1):19-28. DOI: 10.1007/BF00352222. View

18.

Shinohara A, Ogawa T . Homologous recombination and the roles of double-strand breaks. Trends Biochem Sci. 1995; 20(10):387-91. DOI: 10.1016/s0968-0004(00)89085-4. View

19.

Shen Z, Cloud K, Chen D, Park M . Specific interactions between the human RAD51 and RAD52 proteins. J Biol Chem. 1996; 271(1):148-52. DOI: 10.1074/jbc.271.1.148. View

20.

Plug A, Xu J, Reddy G, Golub E, Ashley T . Presynaptic association of Rad51 protein with selected sites in meiotic chromatin. Proc Natl Acad Sci U S A. 1996; 93(12):5920-4. PMC: 39163. DOI: 10.1073/pnas.93.12.5920. View