» Articles » PMID: 11245266

Effect of Cooling of Equine Spermatozoa Before Freezing on Post-thaw Motility: Preliminary Results

Overview
Journal Theriogenology
Publisher Elsevier
Date 2001 Mar 14
PMID 11245266
Citations 5
Authors
Affiliations
Soon will be listed here.
Abstract

The ability to ship cooled stallion semen to a facility that specializes in cryopreservation of spermatozoa would permit stallions to remain at home while their semen is cryopreserved at facilities having the equipment and expertise to freeze the semen properly. To accomplish this goal, methods must be developed to freeze cooled shipped semen. Three experiments were conducted to determine the most appropriate spermatozoal extender, package, time of centrifugation, spermatozoal concentration and length of time after collection that spermatozoa can be cooled before cryopreservation. In the first experiment, spermatozoa were centrifuged to remove seminal plasma, resuspended in either a skim milk extender, a skim milk-egg yolk-sugar extender or a skim milk-egg yolk-salt extender, cooled to 5 degreesC and frozen in 0.5- or 2.5-mL straws either 2.5 or 24 h after cooling. Samples frozen 2.5 h after cooling had higher percentages of progressively motile (PM) spermatozoa (27%) than samples frozen 24 h after cooling (10%; P < 0.05). Samples frozen 2.5 h after cooling in skim milk extenders containing egg yolk had higher percentages of PM spermatozoa (average 32%) than did spermatozoa frozen in extender containing skim milk alone (average 16%; P < 0.05). The percentages of PM spermatozoa frozen in 0.5- or 2.5-mL straws were similar (21 and 28%, respectively; P > 0.05). In the second experiment, spermatozoa were centrifuged to remove seminal plasma either before (25 degreesC) or after cooling (5 degreesC), and spermatozoa were frozen after being cooled to 5 degreesC for 2, 6, or 12 h. The percentages of PM spermatozoa were higher (P < 0.05) for spermatozoa centrifuged before cooling (30%) than for spermatozoa centrifuged after cooling (19%). Spermatozoa centrifuged at 25 degreesC then cooled for 12 h to 5 degreesC had higher (P < 0.05) post-thaw progressive motility (23%) compared to spermatozoa cooled for 12 h and centrifuged at 5 degreesC (13%). In the third experiment, spermatozoa were centrifuged for seminal plasma removal, resuspended at spermatozoal concentrations of 50,250 or 500 x 10(6)/mL, cooled to 5 degreesC for 12 h and then frozen. Samples with spermatozoa packaged at 50 or 250 x 10(6)/mL had higher (P < 0.05 percentages of PM spermatozoa (25 and 23%) after freezing than did samples packaged at 500 x 10(6) spermatozoa/mL (17%). We recommend that semen be centrifuged at 25 degreesC to remove seminal plasma, suspended to 250 x 10(6) spermatozoa/ml and held at 5 degreesC for 12 h prior to freezing.

Citing Articles

A multivariate model for the prediction of pregnancy following laparoscopic artificial insemination of sheep.

Spanner E, de Graaf S, Rickard J Sci Rep. 2024; 14(1):27556.

PMID: 39528692 PMC: 11555048. DOI: 10.1038/s41598-024-79253-x.


Cryopreserving Rabbit Semen: Impact of Varying Sperm Concentrations on Quality and the Standardization of Protocol.

Di Iorio M, Lauriola F, Rusco G, Antenucci E, Schiavitto M, Iaffaldano N Vet Sci. 2024; 11(1).

PMID: 38250915 PMC: 10818829. DOI: 10.3390/vetsci11010009.


Freezability of Dog Semen after Collection in Field Conditions and Cooled Transport.

Colombo M, Morselli M, Franchi G, Schafer-Somi S, Luvoni G Animals (Basel). 2022; 12(7).

PMID: 35405806 PMC: 8997147. DOI: 10.3390/ani12070816.


Centrifugal force assessment in ram sperm: identifying species-specific impact.

Neila-Montero M, Riesco M, Alvarez M, Montes-Garrido R, Boixo J, de Paz P Acta Vet Scand. 2021; 63(1):42.

PMID: 34736507 PMC: 8567708. DOI: 10.1186/s13028-021-00609-8.


Effects of Three Semen Extenders, Breeding Season Month and Freezing-Thawing Cycle on Spermatozoa Preservation of Portuguese Merino Sheep.

Fernandes M, Hernandez P, Simoes J, Barbas J Animals (Basel). 2021; 11(9).

PMID: 34573585 PMC: 8467096. DOI: 10.3390/ani11092619.