Selection for Transgene Homozygosity in Embryonic Stem Cells Results in Extensive Loss of Heterozygosity

Overview

Journal Nat Genet

Specialty Genetics

Date 2001 Mar 10

PMID 11242104

Citations 18

Authors

L Lefebvre

N Dionne

J Karaskova

J A Squire

A Nagy

Affiliations

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Abstract

Embryonic stem cells offer unprecedented opportunities for random or targeted genome alterations in the mouse. We present here an efficient strategy to create chromosome-specific loss of heterozygosity in embryonic stem cells. The combination of this method with genome-wide mutagenesis in ES cells (using chemical mutagens or gene-trap vectors) opens up the possibility for in vitro or in vivo functional screening of recessive mutations in the mouse.

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