Presence of Neurophysin and Vasopressin in the Hypothalamic Magnocellular Nuclei of Rats Homozygous and Heterozygous for Diabetes Insipidus (Brattleboro Strain) As Revealed by Immunoperoxidase History

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1975 Jan 1

PMID 1122531

Citations 8

Authors

W B Watkins

Affiliations

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Abstract

The 3-layer immunoperoxidase-bridge technique was used to study the distribution of neurophysin and vasopressin in the neurosecretory neurons of rats homozygous and heterozygous for diabetes insipidus (Brattleboro strain). In the homozygous rats there was a marked hypertrophy of the hypothalamic magnocellular structures when stained either for neurosecretory material or neurophysin-like antigens. Neurophysin was present in both the paraventricular (PVN) and supraoptic nuclei (SON) of homozygous and heterozygous animals. Less than half of the cells in the PVN and SON were stained for neurophysin. This observation was less apparent when histochemical stains were used to visualize the distribution of neurosecretory material. Although it is generally considered that the homozygous Brattleboro rat does not synthesize vasopressin, a positive reaction was observed in the PVN and SON when anti-[8-lysine]-vasopressin serum was employed in the immunohistochemical procedure.

Citing Articles

Immuno-cytochemical demonstration of the inability of the homozygous Brattleboro rat to synthesize vasopressin and vasopressin-associated neurophysin.

VANDESANDE F, Dierickx K Cell Tissue Res. 1976; 165(3):307-16.

PMID: 1248028 DOI: 10.1007/BF00222435.

Time of fixation and the localization of Gomori-positive and neurophysin-containing structures in the rat hypothalamus.

Watkins W Cell Tissue Res. 1975; 162(4):523-30.

PMID: 1102105 DOI: 10.1007/BF00209351.

Influence of adrenalectomy, total body x-irradiation and dexamethasone on the amount of CRF-granules and "classical" neurosecretory material in the rat neurohypophysis.

Schwabedal P, Bock R Anat Embryol (Berl). 1975; 148(3):267-78.

PMID: 1083173 DOI: 10.1007/BF00319847.

Influence of adrenalectomy on "Gomori-positive" substances in the hypothalamo-neurohypophysial system of rats heterozygous and homozygous for hypothalamic diabetes insipidus.

Schwabedal P, Bock R, Watkins W, Mohring J Anat Embryol (Berl). 1977; 151(1):81-9.

PMID: 907199 DOI: 10.1007/BF00315300.

Cytochemical duality of neurosecretory material in the hypothalamo-posthypophysial system of the rat as related to hormonal content.

Tasso F, Rua S, Picard D Cell Tissue Res. 1977; 180(1):11-29.

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