Permeability Characteristics of Human Endothelial Monolayers Seeded on Different Extracellular Matrix Proteins

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2001 Feb 24

PMID 11200364

Citations 20

Authors

A Nooteboom

T Hendriks

I Otteholler

C J van der Linden

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate whether endothelial monolayer permeability changes induced by inflammatory mediators are affected by the extracellular matrix protein used for cell seeding.

Methods: Human umbilical venular endothelial cells (HUVEC) were grown to confluent monolayers on membranes coated with either collagen, fibronectin or gelatin. The permeability to albumin and dextran was then assessed, both under normal conditions and after treatment with tumor necrosis factor-alpha (TNF-alpha) and bacterial lipopolysaccharide (LPS).

Results: With any of the three protein coatings, tight junctions were formed all over the monolayers. The permeability of the coated membranes to albumin and dextran was reduced strongly by confluent monolayers; the relative reduction was similar for the three matrix proteins used. Pre-incubation of the monolayers with either TNF-alpha or LPS increased permeability dose dependently. However, the relative increase due to either treatment was independent of the protein used for membrane coating.

Conclusion: The extracellular matrix protein used for initial seeding of endothelial cultures plays a minor role in determining the permeability changes induced in HUVEC monolayers by inflammatory mediators.

Citing Articles

Exploring Surface-Enhanced Raman Spectroscopy of Pyrazine-2-Carbonitrile for Indirect Label-Free Albumin Quantification in an Endothelium Permeability Assay.

Klement W, Duijnstee D, Telle V, Staykov A, Browne W, Verpoorte E Anal Chem. 2025; 97(7):4075-4083.

PMID: 39928859 PMC: 11866285. DOI: 10.1021/acs.analchem.4c05906.

Cell-Free Hemoglobin Does Not Attenuate the Effects of SARS-CoV-2 Spike Protein S1 Subunit in Pulmonary Endothelial Cells.

Jana S, Heaven M, Alayash A Int J Mol Sci. 2021; 22(16).

PMID: 34445747 PMC: 8396564. DOI: 10.3390/ijms22169041.

A spatial-institutional analysis of researchers with multiple affiliations.

Yegros-Yegros A, Capponi G, Frenken K PLoS One. 2021; 16(6):e0253462.

PMID: 34185774 PMC: 8241029. DOI: 10.1371/journal.pone.0253462.

SARS-CoV-2, ACE2, and Hydroxychloroquine: Cardiovascular Complications, Therapeutics, and Clinical Readouts in the Current Settings.

Kalra R, Tomar D, Meena A, Kandimalla R Pathogens. 2020; 9(7).

PMID: 32645974 PMC: 7400328. DOI: 10.3390/pathogens9070546.

Neutrophil elastase contributes to the pathological vascular permeability characteristic of diabetic retinopathy.

Liu H, Lessieur E, Saadane A, Lindstrom S, Taylor P, Kern T Diabetologia. 2019; 62(12):2365-2374.

PMID: 31612267 PMC: 6866660. DOI: 10.1007/s00125-019-04998-4.

References

Ghitescu L, Bendayan M . Transendothelial transport of serum albumin: a quantitative immunocytochemical study. J Cell Biol. 1992; 117(4):745-55. PMC: 2289456. DOI: 10.1083/jcb.117.4.745. View

Defilippi P, Silengo L, Tarone G . Alpha 6.beta 1 integrin (laminin receptor) is down-regulated by tumor necrosis factor alpha and interleukin-1 beta in human endothelial cells. J Biol Chem. 1992; 267(26):18303-7. View

Conforti G, Dominguez-Jimenez C, Ronne E, Hoyer-Hansen G, Dejana E . Cell-surface plasminogen activation causes a retraction of in vitro cultured human umbilical vein endothelial cell monolayer. Blood. 1994; 83(4):994-1005. View

Goldblum S, Brann T, Ding X, Pugin J, Tobias P . Lipopolysaccharide (LPS)-binding protein and soluble CD14 function as accessory molecules for LPS-induced changes in endothelial barrier function, in vitro. J Clin Invest. 1994; 93(2):692-702. PMC: 293903. DOI: 10.1172/JCI117022. View

Bradley J, Wilks D, Rubenstein D . The vascular endothelium in septic shock. J Infect. 1994; 28(1):1-10. DOI: 10.1016/s0163-4453(94)93975-6. View

Lampugnani M, Corada M, Caveda L, Breviario F, Ayalon O, Geiger B . The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha-catenin with vascular endothelial cadherin (VE-cadherin). J Cell Biol. 1995; 129(1):203-17. PMC: 2120375. DOI: 10.1083/jcb.129.1.203. View

Ishii Y, Shuyi W, Kitamura S . Soluble CD14 in serum mediates LPS-induced increase in permeability of bovine pulmonary arterial endothelial cell monolayers in vitro. Life Sci. 1995; 56(25):2263-72. DOI: 10.1016/0024-3205(95)00216-s. View

Dejana E, Corada M, Lampugnani M . Endothelial cell-to-cell junctions. FASEB J. 1995; 9(10):910-8. View

Yao J, Bone R, Sawhney R . Differential effects of tumor necrosis factor-alpha on the expression of fibronectin and collagen genes in cultured bovine endothelial cells. Cell Mol Biol Res. 1995; 41(1):17-28. View

10.

Ehringer W, Edwards M, MILLER F . Mechanisms of alpha-thrombin, histamine, and bradykinin induced endothelial permeability. J Cell Physiol. 1996; 167(3):562-9. DOI: 10.1002/(SICI)1097-4652(199606)167:3<562::AID-JCP20>3.0.CO;2-4. View

11.

Kazakoff P, McGuire T, Hoie E, Cano M, Iversen P . An in vitro model for endothelial permeability: assessment of monolayer integrity. In Vitro Cell Dev Biol Anim. 1995; 31(11):846-52. DOI: 10.1007/BF02634568. View

12.

Dejana E . Endothelial adherens junctions: implications in the control of vascular permeability and angiogenesis. J Clin Invest. 1996; 98(9):1949-53. PMC: 507636. DOI: 10.1172/JCI118997. View

13.

Garcia J, Verin A, Schaphorst K . Regulation of thrombin-mediated endothelial cell contraction and permeability. Semin Thromb Hemost. 1996; 22(4):309-15. DOI: 10.1055/s-2007-999025. View

14.

Boyle Jr E, Pohlman T, Johnson M, Verrier E . Endothelial cell injury in cardiovascular surgery: the systemic inflammatory response. Ann Thorac Surg. 1997; 63(1):277-84. DOI: 10.1016/s0003-4975(96)01061-2. View

15.

Davies M, HAGEN P . Systemic inflammatory response syndrome. Br J Surg. 1997; 84(7):920-35. DOI: 10.1002/bjs.1800840707. View

16.

Kusama T, Kohno H, Idezawa T, Fujii H, Yamamoto M, Matsumoto Y . Inhibition of tumor necrosis factor alpha-induced vascular endothelial permeability by gadolinium chloride. Eur Surg Res. 1997; 29(5):375-81. DOI: 10.1159/000129546. View

17.

Tiruppathi C, Song W, Bergenfeldt M, Sass P, Malik A . Gp60 activation mediates albumin transcytosis in endothelial cells by tyrosine kinase-dependent pathway. J Biol Chem. 1997; 272(41):25968-75. DOI: 10.1074/jbc.272.41.25968. View

18.

Gulino D, Delachanal E, Concord E, Genoux Y, Morand B, Valiron M . Alteration of endothelial cell monolayer integrity triggers resynthesis of vascular endothelium cadherin. J Biol Chem. 1998; 273(45):29786-93. DOI: 10.1074/jbc.273.45.29786. View

19.

Bannerman D, Sathyamoorthy M, Goldblum S . Bacterial lipopolysaccharide disrupts endothelial monolayer integrity and survival signaling events through caspase cleavage of adherens junction proteins. J Biol Chem. 1998; 273(52):35371-80. DOI: 10.1074/jbc.273.52.35371. View

20.

Jaffe E, Nachman R, Becker C, Minick C . Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973; 52(11):2745-56. PMC: 302542. DOI: 10.1172/JCI107470. View