Mechanical Transmission of Poleroviruses

Overview

Journal J Virol Methods

Specialty Microbiology

Date 2001 Feb 13

PMID 11164501

Citations 5

Authors

K Hoffmann

M Verbeek

A Romano

A M Dullemans

J F van den Heuvel

F van der Wilk

Affiliations

Soon will be listed here.

Abstract

Previously, transmission of poleroviruses has relied solely on the use of their aphid vectors. Biolistic inoculation allowed for the first time the mechanical transmission of Beet western yellows virus (BWYV) and Potato leafroll virus (PLRV) to several host plants. Inoculation with purified preparations and viral RNA extracts of PLRV resulted in 30-50% systemically infected Nicotiana occidentalis P1 plants and 15-30% infected Nicotiana clevelandii plants. Particle bombardment was also used successfully to infect N. clevelandii plants with in vitro RNA transcripts of full-length cDNA of BWYV.

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A simple, novel and high efficiency sap inoculation method to screen for tobacco streak virus.

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