CD40-CD40L Independent Ig Gene Hypermutation Suggests a Second B Cell Diversification Pathway in Humans

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2001 Feb 7

PMID 11158612

Citations 142

Authors

S Weller

A Faili

C Garcia

M C Braun

F Le Deist F

G de Saint Basile G

O Hermine

A Fischer

C A REYNAUD

J C Weill

Affiliations

Soon will be listed here.

Abstract

Somatically mutated IgM(+)-only and IgM(+)IgD(+)CD27(+) B lymphocytes comprise approximately 25% of the human peripheral B cell pool. These cells phenotypically resemble class-switched B cells and have therefore been classified as postgerminal center memory B cells. X-linked hyper IgM patients have a genetic defect characterized by a mutation of the CD40L gene. These patients, who do not express a functional CD40 ligand, cannot switch Ig isotypes and do not form germinal centers and memory B cells. We report here that an IgM(+)IgD(+)CD27(+) B cell subset with somatically mutated Ig receptors is generated in these patients, implying that these cells expand and diversify their Ig receptors in the absence of classical cognate T-B collaboration. The presence of this sole subset in the absence of IgM(+)-only and switched CD27(+) memory B cells suggests that it belongs to a separate diversification pathway.

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