Characteristics of the Inhibitory Effect of Calmodulin on Specific [125i]omega-conotoxin GVIA Binding to Crude Membranes from Chick Brain

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2001 Jan 11

PMID 11152392

Citations 5

Authors

S Ichida

J Abe

Y A Zhang

K Sugihara

K Imoto

T Wada

N Fujita

H Sohma

Affiliations

Soon will be listed here.

Abstract

The characteristics of the inhibitory effect of calcium ion (Ca2+)/calmodulin (CaM) on specific [125I]-omega-conotoxin GVIA (125I-omega-CTX) binding and on the labeling of 125I-omega-CTX to crude membranes from chick brain were investigated. The inhibitory effect of Ca2+/CaM depended on the concentrations of free Ca2+ and CaM. The IC50 values for free Ca2+ and CaM were about 2.0 x 10(-8) M and 3.0 microg protein/ml, respectively. The inhibitory effect of Ca2+/CaM was attenuated by the CaM antagonists W-7, prenylamine and CaM-kinase II fragment (290-309), but not by the calcineurin inhibitor FK506. Ca2+/CaM also inhibited the labeling of a 135-kDa band (which was considered to be part of N-type Ca2+ channel alpha1 subunits) with 125I-omega-CTX using a cross-linker. These results suggest that Ca2+/CaM affects specific 125I-omega-CTX binding sites, probably N-type Ca2+ channel alpha1 subunits, in crude membranes from chick whole brain.

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References

Luan Y, Matsuura I, Yazawa M, Nakamura T, Yagi K . Yeast calmodulin: structural and functional differences compared with vertebrate calmodulin. J Biochem. 1987; 102(6):1531-7. DOI: 10.1093/oxfordjournals.jbchem.a122201. View

Hess P . Calcium channels in vertebrate cells. Annu Rev Neurosci. 1990; 13:337-56. DOI: 10.1146/annurev.ne.13.030190.002005. View

Chen T, Yau K . Direct modulation by Ca(2+)-calmodulin of cyclic nucleotide-activated channel of rat olfactory receptor neurons. Nature. 1994; 368(6471):545-8. DOI: 10.1038/368545a0. View

Feigenbaum P, Garcia M, Kaczorowski G . Evidence for distinct sites coupled to high affinity omega-conotoxin receptors in rat brain synaptic plasma membrane vesicles. Biochem Biophys Res Commun. 1988; 154(1):298-305. DOI: 10.1016/0006-291x(88)90684-5. View

Ichida S, Wada T, Sekiguchi M, Kishino H, Okazaki Y, Akimoto T . Characteristics of specific 125I-omega-conotoxin GVIA binding in rat whole brain. Neurochem Res. 1993; 18(11):1137-44. DOI: 10.1007/BF00978364. View

Herlitze S, Garcia D, Mackie K, Hille B, Scheuer T, Catterall W . Modulation of Ca2+ channels by G-protein beta gamma subunits. Nature. 1996; 380(6571):258-62. DOI: 10.1038/380258a0. View

Saimi Y, Ling K . Calmodulin activation of calcium-dependent sodium channels in excised membrane patches of Paramecium. Science. 1990; 249(4975):1441-4. DOI: 10.1126/science.2169650. View

Ellinor P, Zhang J, Horne W, Tsien R . Structural determinants of the blockade of N-type calcium channels by a peptide neurotoxin. Nature. 1994; 372(6503):272-5. DOI: 10.1038/372272a0. View

Ichida S, Wada T, Akimoto T, Kasamatsu Y, Tahara M, HASIMOTO K . Characteristics of [125I]omega-conotoxin labeling using bifunctional cross linker DSP in crude membranes from chick brain. Neurochem Res. 1995; 20(4):467-73. DOI: 10.1007/BF00973104. View

10.

Olivera B, McIntosh J, Cruz L, Luque F, Gray W . Purification and sequence of a presynaptic peptide toxin from Conus geographus venom. Biochemistry. 1984; 23(22):5087-90. DOI: 10.1021/bi00317a001. View

11.

Ichida S, Wada T, Tahara M, Imoto K, Zhang Y . Relationship between specific binding of 125I-omega-conotoxin GVIA and GTP binding protein: effects of the GTP analogues, mastoparan and A1F4-. Biochim Biophys Acta. 1997; 1325(2):215-25. DOI: 10.1016/s0005-2736(96)00258-1. View

12.

Means A, Tash J, Chafouleas J . Physiological implications of the presence, distribution, and regulation of calmodulin in eukaryotic cells. Physiol Rev. 1982; 62(1):1-39. DOI: 10.1152/physrev.1982.62.1.1. View

13.

McCleskey E, Fox A, Feldman D, Tsien R . Different types of calcium channels. J Exp Biol. 1986; 124:177-90. DOI: 10.1242/jeb.124.1.177. View

14.

Ichida S, Abe J, Sugihara K, Imoto K, Wada T, Sohma H . Calcium/calmodulin inhibits the binding of specific [125I]omega-conotoxin GVIA to chick brain membranes. Neurochem Res. 2000; 25(3):335-40. DOI: 10.1023/a:1007532818918. View

15.

Miller R . Voltage-sensitive Ca2+ channels. J Biol Chem. 1992; 267(3):1403-6. View

16.

Smith J, Rousseau E, Meissner G . Calmodulin modulation of single sarcoplasmic reticulum Ca2+-release channels from cardiac and skeletal muscle. Circ Res. 1989; 64(2):352-9. DOI: 10.1161/01.res.64.2.352. View

17.

Yazawa M, Ikura M, Hikichi K, Ying L, Yagi K . Communication between two globular domains of calmodulin in the presence of mastoparan or caldesmon fragment. Ca2+ binding and 1H NMR. J Biol Chem. 1987; 262(23):10951-4. View

18.

Koyano K, Abe T, Nishiuchi Y, Sakakibara S . Effects of synthetic omega-conotoxin on synaptic transmission. Eur J Pharmacol. 1987; 135(3):337-43. DOI: 10.1016/0014-2999(87)90683-2. View

19.

Oyama Y, Tsuda Y, Sakakibara S, Akaike N . Synthetic omega-conotoxin: a potent calcium channel blocking neurotoxin. Brain Res. 1987; 424(1):58-64. DOI: 10.1016/0006-8993(87)91192-9. View

20.

Olivera B, Miljanich G, Ramachandran J, Adams M . Calcium channel diversity and neurotransmitter release: the omega-conotoxins and omega-agatoxins. Annu Rev Biochem. 1994; 63:823-67. DOI: 10.1146/annurev.bi.63.070194.004135. View