RNA Degradosomes Exist in Vivo in Escherichia Coli As Multicomponent Complexes Associated with the Cytoplasmic Membrane Via the N-terminal Region of Ribonuclease E

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2001 Jan 3

PMID 11134527

Citations 67

Authors

G G Liou

W N Jane

S N Cohen

N S Lin

S Lin-Chao

Affiliations

Soon will be listed here.

Abstract

RNase E isolated from Escherichia coli is contained in a multicomponent "degradosome" complex with other proteins implicated in RNA decay. Earlier work has shown that the C-terminal region of RNase E is a scaffold for the binding of degradosome components and has identified specific RNase E segments necessary for its interaction with polynucleotide phosphorylase (PNPase), RhlB RNA helicase, and enolase. Here, we report electron microscopy studies that use immunogold labeling and freeze-fracture methods to show that degradosomes exist in vivo in E. coli as multicomponent structures that associate with the cytoplasmic membrane via the N-terminal region of RNase E. Whereas PNPase and enolase are present in E. coli in large excess relative to RNase E and therefore are detected in cells largely as molecules unlinked to the RNase E scaffold, immunogold labeling and biochemical analyses show that helicase is present in approximately equimolar amounts to RNase E at all cell growth stages. Our findings, which establish the existence and cellular location of RNase E-based degradosomes in vivo in E. coli, also suggest that RNA processing and decay may occur at specific sites within cells.

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