Recombinant Expression of Lymphocytic Choriomeningitis Virus Strain WE Glycoproteins: a Single Amino Acid Makes the Difference

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2001 Jan 3

PMID 11134321

Citations 19

Authors

W R Beyer

H Miletic

W Ostertag

D von Laer

Affiliations

Soon will be listed here.

Abstract

Cytoplasmic vector systems are generally used for expression of lymphocytic choriomeningitis virus (LCMV) proteins. However, we achieved high levels of cell surface glycoproteins using a standard nuclear expression plasmid. Expression was independent of other LCMV proteins but was blocked by a missense mutation within the original LCMV(WE) glycoprotein cDNA.

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